Primary cilia-regulated transcriptome in the renal collecting duct

Sami G Mohammed, Francisco J Arjona, Eric H J Verschuren, Zeineb Bakey, Wynand Alkema, Sacha van Hijum, Miriam Schmidts, René J M Bindels, Joost G J Hoenderop

Onderzoeksoutput: ArticleAcademicpeer review

Samenvatting

Renal tubular cells respond to mechanical stimuli generated by urinary flow to regulate the activity and transcript abundance of important genes for ion handling, cellular homeostasis, and proper renal development. The primary cilium, a mechanosensory organelle, is postulated to regulate this mRNA response. The aim of this study is to reveal the transcriptome changes of tubular epithelia in response to fluid flow and determine the role of primary cilia in this process. Inner-medullary collecting duct (CD) cells were subjected to either static or physiologically relevant fluid flow (∼0.6 dyn/cm2). RNA-sequencing analysis of ciliated cells subjected to fluid flow showed up-regulation of 1379 genes and down-regulation of 1294 genes compared with static control cells. Strikingly, only 54 of these genes were identified as gene candidates sensitive to primary cilia sensing of fluid flow, of which 16 were linked to ion or water transport pathways in the CD. Validation by quantitative real-time PCR revealed that only the expression of transferrin receptor, which is involved in iron transport; and tribbles pseudokinase 3, which is involved in insulin signaling, were unequivocally regulated by primary cilia sensing of fluid flow. This study shows that the involvement of primary cilia in ion transport in the collecting duct is exceptionally specific.

Originele taal-2English
Pagina's (van-tot)3653-3668
TijdschriftFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume32
Nummer van het tijdschrift7
DOI's
StatusPublished - 8 feb 2018
Extern gepubliceerdJa

Keywords

  • genetica

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  • Citeer dit

    Mohammed, S. G., Arjona, F. J., Verschuren, E. H. J., Bakey, Z., Alkema, W., van Hijum, S., Schmidts, M., Bindels, R. J. M., & Hoenderop, J. G. J. (2018). Primary cilia-regulated transcriptome in the renal collecting duct. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(7), 3653-3668. https://doi.org/10.1096/fj.201701228R