TY - JOUR
T1 - Melt Electrowriting of Polyhydroxyalkanoates for Enzymatically Degradable Scaffolds
AU - Gładysz, Magdalena Z.
AU - Ubels, Didi
AU - Koch, Marcus
AU - Amirsadeghi, Armin
AU - Alleblas, Frederique
AU - Kamperman, Marleen
AU - Siebring, Jeroen
AU - Nagelkerke, Anika
AU - Włodarczyk-Biegun , Małgorzata K.
AU - van Vliet, Sander
PY - 2024/11/12
Y1 - 2024/11/12
N2 - Melt electrowriting (MEW) enables precise scaffold fabrication for biomedical applications. With a limited number of processable materials with short and tunable degradation times, polyhydroxyalkanoates (PHAs) present an interesting option. Here, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a blend of PHBV and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PHBV+P34HB) are successfully melt electrowritten into scaffolds with various architectures. PHBV+P34HB exhibits greater thermal stability, making it a superior printing material compared to PHBV in MEW. The PHBV+P34HB scaffolds subjected to enzymatic degradation show tunable degradation times, governed by enzyme dilution, incubation time, and scaffold surface area. PHBV+P34HB scaffolds seeded with human dermal fibroblasts (HDFs), demonstrate enhanced cell adherence, proliferation, and spreading. The HDFs, when exposed to the enzyme solutions and enzymatic degradation residues, show good viability and proliferation rates. Additionally, HDFs grown on enzymatically pre-incubated scaffolds do not show any difference in behavior compared those grown on control scaffolds. It is concluded that PHAs, as biobased materials with enzymatically tunable degradability rates, are an important addition to the already limited set of materials available for MEW technology.
AB - Melt electrowriting (MEW) enables precise scaffold fabrication for biomedical applications. With a limited number of processable materials with short and tunable degradation times, polyhydroxyalkanoates (PHAs) present an interesting option. Here, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a blend of PHBV and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PHBV+P34HB) are successfully melt electrowritten into scaffolds with various architectures. PHBV+P34HB exhibits greater thermal stability, making it a superior printing material compared to PHBV in MEW. The PHBV+P34HB scaffolds subjected to enzymatic degradation show tunable degradation times, governed by enzyme dilution, incubation time, and scaffold surface area. PHBV+P34HB scaffolds seeded with human dermal fibroblasts (HDFs), demonstrate enhanced cell adherence, proliferation, and spreading. The HDFs, when exposed to the enzyme solutions and enzymatic degradation residues, show good viability and proliferation rates. Additionally, HDFs grown on enzymatically pre-incubated scaffolds do not show any difference in behavior compared those grown on control scaffolds. It is concluded that PHAs, as biobased materials with enzymatically tunable degradability rates, are an important addition to the already limited set of materials available for MEW technology.
KW - enzymatically degradable scaffolds
KW - melt electrowriting
KW - polyhydroxyalkanoates
KW - MEW
KW - enzymatisch afbreekbare steigers
KW - polyhydroxyalkanoaten
U2 - 10.1002/adhm.202401504
DO - 10.1002/adhm.202401504
M3 - Article
SN - 2192-2659
VL - online version of record before inclusion in an issue
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
M1 - 2401504
ER -