Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

J Krooneman, F Faber, A C Alderkamp, S J H W Oude Elferink, F Driehuis, I Cleenwerck, J Swings, J C Gottschal, M Vancanneyt

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Uittreksel

Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T (= DSM 14421T).

Originele taal-2English
Pagina's (van-tot)639-646
TijdschriftInternational journal of systematic and evolutionary microbiology
Volume52
Nummer van het tijdschriftPt 2
DOI's
StatusPublished - mrt 2002

Keywords

  • microbiologie

Citeer dit

Krooneman, J ; Faber, F ; Alderkamp, A C ; Elferink, S J H W Oude ; Driehuis, F ; Cleenwerck, I ; Swings, J ; Gottschal, J C ; Vancanneyt, M. / Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. In: International journal of systematic and evolutionary microbiology. 2002 ; Vol. 52, Nr. Pt 2. blz. 639-646.
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title = "Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage",
abstract = "Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T (= DSM 14421T).",
keywords = "anaerobiosis, biodegradation, environmental, dna, bacterial/chemistry, lactobacillus/classification, molecular sequence data, nucleic acid hybridization, phylogeny, propionates/analysis, propylene glycol/metabolism, rna, ribosomal, 16S/chemistry, silage/microbiology, zea mays/microbiology, microbiologie",
author = "J Krooneman and F Faber and Alderkamp, {A C} and Elferink, {S J H W Oude} and F Driehuis and I Cleenwerck and J Swings and Gottschal, {J C} and M Vancanneyt",
year = "2002",
month = "3",
doi = "10.1099/00207713-52-2-639",
language = "English",
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pages = "639--646",
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Krooneman, J, Faber, F, Alderkamp, AC, Elferink, SJHWO, Driehuis, F, Cleenwerck, I, Swings, J, Gottschal, JC & Vancanneyt, M 2002, 'Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage' International journal of systematic and evolutionary microbiology, vol. 52, nr. Pt 2, blz. 639-646. https://doi.org/10.1099/00207713-52-2-639

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. / Krooneman, J; Faber, F; Alderkamp, A C; Elferink, S J H W Oude; Driehuis, F; Cleenwerck, I; Swings, J; Gottschal, J C; Vancanneyt, M.

In: International journal of systematic and evolutionary microbiology, Vol. 52, Nr. Pt 2, 03.2002, blz. 639-646.

Onderzoeksoutput: ArticleAcademicpeer review

TY - JOUR

T1 - Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

AU - Krooneman, J

AU - Faber, F

AU - Alderkamp, A C

AU - Elferink, S J H W Oude

AU - Driehuis, F

AU - Cleenwerck, I

AU - Swings, J

AU - Gottschal, J C

AU - Vancanneyt, M

PY - 2002/3

Y1 - 2002/3

N2 - Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T (= DSM 14421T).

AB - Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T (= DSM 14421T).

KW - anaerobiosis

KW - biodegradation, environmental

KW - dna, bacterial/chemistry

KW - lactobacillus/classification

KW - molecular sequence data

KW - nucleic acid hybridization

KW - phylogeny

KW - propionates/analysis

KW - propylene glycol/metabolism

KW - rna, ribosomal, 16S/chemistry

KW - silage/microbiology

KW - zea mays/microbiology

KW - microbiologie

UR - http://www.mendeley.com/research/lactobacillus-diolivorans-sp-nov-12propanedioldegrading-bacterium-isolated-aerobically-stable-maize

U2 - 10.1099/00207713-52-2-639

DO - 10.1099/00207713-52-2-639

M3 - Article

VL - 52

SP - 639

EP - 646

JO - International journal of systematic and evolutionary microbiology

JF - International journal of systematic and evolutionary microbiology

SN - 1466-5026

IS - Pt 2

ER -