Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A

Fean D Sarian, Rachel M van der Kaaij, Slavko Kralj, Dirk-Jan Wijbenga, Doede J. Binnema, Marc J E C van der Maarel, Lubbert Dijkhuizen

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Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.

Originele taal-2English
Pagina's (van-tot)645-654
TijdschriftApplied microbiology and biotechnology
Volume93
Nummer van het tijdschrift2
DOI's
StatusPublished - jan 2012

Keywords

    Citeer dit

    Sarian, F. D., van der Kaaij, R. M., Kralj, S., Wijbenga, D-J., Binnema, D. J., van der Maarel, M. J. E. C., & Dijkhuizen, L. (2012). Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A. Applied microbiology and biotechnology, 93(2), 645-654. https://doi.org/10.1007/s00253-011-3436-7
    Sarian, Fean D ; van der Kaaij, Rachel M ; Kralj, Slavko ; Wijbenga, Dirk-Jan ; Binnema, Doede J. ; van der Maarel, Marc J E C ; Dijkhuizen, Lubbert. / Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A. In: Applied microbiology and biotechnology. 2012 ; Vol. 93, Nr. 2. blz. 645-654.
    @article{3bb03d33ec36448388aedfcb1b997dbf,
    title = "Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A",
    abstract = "Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.",
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    author = "Sarian, {Fean D} and {van der Kaaij}, {Rachel M} and Slavko Kralj and Dirk-Jan Wijbenga and Binnema, {Doede J.} and {van der Maarel}, {Marc J E C} and Lubbert Dijkhuizen",
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    doi = "10.1007/s00253-011-3436-7",
    language = "English",
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    Sarian, FD, van der Kaaij, RM, Kralj, S, Wijbenga, D-J, Binnema, DJ, van der Maarel, MJEC & Dijkhuizen, L 2012, 'Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A' Applied microbiology and biotechnology, vol. 93, nr. 2, blz. 645-654. https://doi.org/10.1007/s00253-011-3436-7

    Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A. / Sarian, Fean D; van der Kaaij, Rachel M; Kralj, Slavko; Wijbenga, Dirk-Jan; Binnema, Doede J.; van der Maarel, Marc J E C; Dijkhuizen, Lubbert.

    In: Applied microbiology and biotechnology, Vol. 93, Nr. 2, 01.2012, blz. 645-654.

    Onderzoeksoutput: ArticleAcademicpeer review

    TY - JOUR

    T1 - Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A

    AU - Sarian, Fean D

    AU - van der Kaaij, Rachel M

    AU - Kralj, Slavko

    AU - Wijbenga, Dirk-Jan

    AU - Binnema, Doede J.

    AU - van der Maarel, Marc J E C

    AU - Dijkhuizen, Lubbert

    PY - 2012/1

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    N2 - Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.

    AB - Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.

    KW - Actinomycetales

    KW - Cluster Analysis

    KW - DNA, Bacterial

    KW - DNA, Ribosomal

    KW - Maltose

    KW - Manihot

    KW - Molecular Sequence Data

    KW - Oligosaccharides

    KW - Phylogeny

    KW - RNA, Ribosomal, 16S

    KW - Sequence Analysis, DNA

    KW - Solanum tuberosum

    KW - Starch

    KW - Temperature

    KW - Trisaccharides

    KW - Triticum

    KW - Journal Article

    KW - Research Support, Non-U.S. Gov't

    U2 - 10.1007/s00253-011-3436-7

    DO - 10.1007/s00253-011-3436-7

    M3 - Article

    VL - 93

    SP - 645

    EP - 654

    JO - Applied microbiology and biotechnology

    JF - Applied microbiology and biotechnology

    SN - 0175-7598

    IS - 2

    ER -