Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri

S J Oude Elferink, J Krooneman, J C Gottschal, S F Spoelstra, F Faber, F Driehuis

Onderzoeksoutput: ArticleAcademicpeer review

Uittreksel

The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic acid under anoxic conditions, without requiring an external electron acceptor. Each mole of lactic acid was converted into approximately 0.5 mol of acetic acid, 0.5 mol of 1,2-propanediol, and traces of ethanol. Based on stoichiometry studies and the high levels of NAD-linked 1, 2-propanediol-dependent oxidoreductase (530 to 790 nmol min(-1) mg of protein(-1)), a novel pathway for anaerobic lactic acid degradation is proposed. The anaerobic degradation of lactic acid by L. buchneri does not support cell growth and is pH dependent. Acidic conditions are needed to induce the lactic-acid-degrading capacity of the cells and to maintain the lactic-acid-degrading activity. At a pH above 5.8 hardly any lactic acid degradation was observed. The exact function of anaerobic lactic acid degradation by L. buchneri is not certain, but some results indicate that it plays a role in maintaining cell viability.

Originele taal-2English
Pagina's (van-tot)125-132
TijdschriftApplied and environmental microbiology
Volume67
Nummer van het tijdschrift1
DOI's
StatusPublished - jan 2001

Keywords

  • melkzuur

Citeer dit

Oude Elferink, S J ; Krooneman, J ; Gottschal, J C ; Spoelstra, S F ; Faber, F ; Driehuis, F. / Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri. In: Applied and environmental microbiology. 2001 ; Vol. 67, Nr. 1. blz. 125-132.
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title = "Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri",
abstract = "The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic acid under anoxic conditions, without requiring an external electron acceptor. Each mole of lactic acid was converted into approximately 0.5 mol of acetic acid, 0.5 mol of 1,2-propanediol, and traces of ethanol. Based on stoichiometry studies and the high levels of NAD-linked 1, 2-propanediol-dependent oxidoreductase (530 to 790 nmol min(-1) mg of protein(-1)), a novel pathway for anaerobic lactic acid degradation is proposed. The anaerobic degradation of lactic acid by L. buchneri does not support cell growth and is pH dependent. Acidic conditions are needed to induce the lactic-acid-degrading capacity of the cells and to maintain the lactic-acid-degrading activity. At a pH above 5.8 hardly any lactic acid degradation was observed. The exact function of anaerobic lactic acid degradation by L. buchneri is not certain, but some results indicate that it plays a role in maintaining cell viability.",
keywords = "acetic acid/metabolism, anaerobiosis, biodegradation, environmental, culture media, hydrogen-ion concentration, lactic acid/metabolism, lactobacillus/growth & development, nadh dehydrogenase/metabolism, propylene glycol/metabolism, temperature, melkzuur",
author = "{Oude Elferink}, {S J} and J Krooneman and Gottschal, {J C} and Spoelstra, {S F} and F Faber and F Driehuis",
year = "2001",
month = "1",
doi = "10.1128/AEM.67.1.125-132.2001",
language = "English",
volume = "67",
pages = "125--132",
journal = "Applied and environmental microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
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Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri. / Oude Elferink, S J; Krooneman, J; Gottschal, J C; Spoelstra, S F; Faber, F; Driehuis, F.

In: Applied and environmental microbiology, Vol. 67, Nr. 1, 01.2001, blz. 125-132.

Onderzoeksoutput: ArticleAcademicpeer review

TY - JOUR

T1 - Anaerobic conversion of lactic acid to acetic acid and 1, 2-propanediol by Lactobacillus buchneri

AU - Oude Elferink, S J

AU - Krooneman, J

AU - Gottschal, J C

AU - Spoelstra, S F

AU - Faber, F

AU - Driehuis, F

PY - 2001/1

Y1 - 2001/1

N2 - The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic acid under anoxic conditions, without requiring an external electron acceptor. Each mole of lactic acid was converted into approximately 0.5 mol of acetic acid, 0.5 mol of 1,2-propanediol, and traces of ethanol. Based on stoichiometry studies and the high levels of NAD-linked 1, 2-propanediol-dependent oxidoreductase (530 to 790 nmol min(-1) mg of protein(-1)), a novel pathway for anaerobic lactic acid degradation is proposed. The anaerobic degradation of lactic acid by L. buchneri does not support cell growth and is pH dependent. Acidic conditions are needed to induce the lactic-acid-degrading capacity of the cells and to maintain the lactic-acid-degrading activity. At a pH above 5.8 hardly any lactic acid degradation was observed. The exact function of anaerobic lactic acid degradation by L. buchneri is not certain, but some results indicate that it plays a role in maintaining cell viability.

AB - The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic acid under anoxic conditions, without requiring an external electron acceptor. Each mole of lactic acid was converted into approximately 0.5 mol of acetic acid, 0.5 mol of 1,2-propanediol, and traces of ethanol. Based on stoichiometry studies and the high levels of NAD-linked 1, 2-propanediol-dependent oxidoreductase (530 to 790 nmol min(-1) mg of protein(-1)), a novel pathway for anaerobic lactic acid degradation is proposed. The anaerobic degradation of lactic acid by L. buchneri does not support cell growth and is pH dependent. Acidic conditions are needed to induce the lactic-acid-degrading capacity of the cells and to maintain the lactic-acid-degrading activity. At a pH above 5.8 hardly any lactic acid degradation was observed. The exact function of anaerobic lactic acid degradation by L. buchneri is not certain, but some results indicate that it plays a role in maintaining cell viability.

KW - acetic acid/metabolism

KW - anaerobiosis

KW - biodegradation, environmental

KW - culture media

KW - hydrogen-ion concentration

KW - lactic acid/metabolism

KW - lactobacillus/growth & development

KW - nadh dehydrogenase/metabolism

KW - propylene glycol/metabolism

KW - temperature

KW - melkzuur

U2 - 10.1128/AEM.67.1.125-132.2001

DO - 10.1128/AEM.67.1.125-132.2001

M3 - Article

VL - 67

SP - 125

EP - 132

JO - Applied and environmental microbiology

JF - Applied and environmental microbiology

SN - 0099-2240

IS - 1

ER -