WP 2C; analysis of enzymatically modified starch

Monique Ebbelaar

Research output: Contribution to conferencePosterOther research output

Abstract

ALIFE:The “as eaten” method to measure the Total Dietary Fibre content was implemented at the Hanze University of Applied Sciences (WP 4). The enzymatic treatment with the GtfB enzyme clearly resulted in an increased fibre content of starch from 1.6% to approx. 20% (fig A). When using this modified starch (“as eaten” treated) in an incubation with colon bacteria we see a similar pattern as when using FOS and GOS (fig B). The qPCR results indicate a significant stimulation of the growth of gut bacteria by the GtfB modified starch, as shown by the relative increase of Bacteroides and to a lesser extent Lactobacilli (fig.C). The prebiotic effect remains to be evaluated.
Original languageEnglish
Number of pages1
Publication statusPublished - 16 Apr 2012
EventSymposium Carbohydrate Competence Center (CCC) - Hampshire Plaza, Groningen, Netherlands
Duration: 26 Apr 2012 → …
http://www.rug.nl/news/2012/04/cccuitn

Conference

ConferenceSymposium Carbohydrate Competence Center (CCC)
CountryNetherlands
CityGroningen
Period26/04/12 → …
Internet address

Keywords

  • modified starch
  • dietary fiber
  • prebiotic

Cite this

Ebbelaar, M. (2012). WP 2C; analysis of enzymatically modified starch. Poster session presented at Symposium Carbohydrate Competence Center (CCC), Groningen, Netherlands.
Ebbelaar, Monique. / WP 2C; analysis of enzymatically modified starch. Poster session presented at Symposium Carbohydrate Competence Center (CCC), Groningen, Netherlands.1 p.
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Ebbelaar, M 2012, 'WP 2C; analysis of enzymatically modified starch' Symposium Carbohydrate Competence Center (CCC), Groningen, Netherlands, 26/04/12, .

WP 2C; analysis of enzymatically modified starch. / Ebbelaar, Monique.

2012. Poster session presented at Symposium Carbohydrate Competence Center (CCC), Groningen, Netherlands.

Research output: Contribution to conferencePosterOther research output

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T1 - WP 2C; analysis of enzymatically modified starch

AU - Ebbelaar, Monique

PY - 2012/4/16

Y1 - 2012/4/16

N2 - ALIFE:The “as eaten” method to measure the Total Dietary Fibre content was implemented at the Hanze University of Applied Sciences (WP 4). The enzymatic treatment with the GtfB enzyme clearly resulted in an increased fibre content of starch from 1.6% to approx. 20% (fig A). When using this modified starch (“as eaten” treated) in an incubation with colon bacteria we see a similar pattern as when using FOS and GOS (fig B). The qPCR results indicate a significant stimulation of the growth of gut bacteria by the GtfB modified starch, as shown by the relative increase of Bacteroides and to a lesser extent Lactobacilli (fig.C). The prebiotic effect remains to be evaluated.

AB - ALIFE:The “as eaten” method to measure the Total Dietary Fibre content was implemented at the Hanze University of Applied Sciences (WP 4). The enzymatic treatment with the GtfB enzyme clearly resulted in an increased fibre content of starch from 1.6% to approx. 20% (fig A). When using this modified starch (“as eaten” treated) in an incubation with colon bacteria we see a similar pattern as when using FOS and GOS (fig B). The qPCR results indicate a significant stimulation of the growth of gut bacteria by the GtfB modified starch, as shown by the relative increase of Bacteroides and to a lesser extent Lactobacilli (fig.C). The prebiotic effect remains to be evaluated.

KW - gemodificeerd zetmeel

KW - voedingsvezels

KW - prebiotic

KW - modified starch

KW - dietary fiber

KW - prebiotic

M3 - Poster

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Ebbelaar M. WP 2C; analysis of enzymatically modified starch. 2012. Poster session presented at Symposium Carbohydrate Competence Center (CCC), Groningen, Netherlands.