Abstract
Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.
| Original language | English |
|---|---|
| Pages (from-to) | 869-871 |
| Journal | Transgenic Research |
| Volume | 22 |
| Issue number | 4 |
| Publication status | Published - Aug 2013 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 07 Affordable and Clean Energy -
SDG 09 Industry, Innovation, and Infrastructure
Keywords
- cloning
- transgene inactivation
- arabidopsis thaliana
Research Focus Areas Hanze University of Applied Sciences * (mandatory by Hanze)
- Energy
Research Focus Areas Research Centre or Centre of Expertise * (mandatory by Hanze)
- Renewable fuels and sustainable gases
Publinova themes
- Technology
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