Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene

Jan Peter Nap, Karol J. Rogowski, Adam Folta, Joachim W. Bargsten, Ludmila Mlynarova

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Abstract

Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.
Original languageEnglish
Pages (from-to)869-871
JournalTransgenic research
Volume22
Issue number4
Publication statusPublished - Aug 2013

Keywords

  • cloning
  • transgene inactivation
  • arabidopsis thaliana

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    Nap, J. P., Rogowski, K. J., Folta, A., Bargsten, J. W., & Mlynarova, L. (2013). Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene. Transgenic research, 22(4), 869-871.