Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene

Jan Peter Nap, Karol J. Rogowski, Adam Folta, Joachim W. Bargsten, Ludmila Mlynarova

Research output: Contribution to journalArticleAcademicpeer-review

17 Downloads (Pure)

Abstract

Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.
Original languageEnglish
Pages (from-to)869-871
JournalTransgenic research
Volume22
Issue number4
Publication statusPublished - Aug 2013

Keywords

  • cloning
  • transgene inactivation
  • arabidopsis thaliana

Cite this

Nap, J. P., Rogowski, K. J., Folta, A., Bargsten, J. W., & Mlynarova, L. (2013). Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene. Transgenic research, 22(4), 869-871.
Nap, Jan Peter ; Rogowski, Karol J. ; Folta, Adam ; Bargsten, Joachim W. ; Mlynarova, Ludmila. / Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene. In: Transgenic research. 2013 ; Vol. 22, No. 4. pp. 869-871.
@article{3de5c074c41f4a438e07085b8cae576b,
title = "Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene",
abstract = "Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.",
keywords = "cloning, transgene inactivation, arabidopsis thaliana, klonen",
author = "Nap, {Jan Peter} and Rogowski, {Karol J.} and Adam Folta and Bargsten, {Joachim W.} and Ludmila Mlynarova",
year = "2013",
month = "8",
language = "English",
volume = "22",
pages = "869--871",
journal = "Transgenic research",
issn = "0962-8819",
publisher = "Springer Verlag",
number = "4",

}

Nap, JP, Rogowski, KJ, Folta, A, Bargsten, JW & Mlynarova, L 2013, 'Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene' Transgenic research, vol. 22, no. 4, pp. 869-871.

Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene. / Nap, Jan Peter; Rogowski, Karol J.; Folta, Adam; Bargsten, Joachim W.; Mlynarova, Ludmila.

In: Transgenic research, Vol. 22, No. 4, 08.2013, p. 869-871.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene

AU - Nap, Jan Peter

AU - Rogowski, Karol J.

AU - Folta, Adam

AU - Bargsten, Joachim W.

AU - Mlynarova, Ludmila

PY - 2013/8

Y1 - 2013/8

N2 - Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.

AB - Common cloning is often associated with instability of certain classes of DNA. Here we report on IS1 transposition as possible source of such instability. During the cloning of Arabidopsis thaliana gene into commercially available vector maintained in widely used Escherichia coli host the insertion of complete IS1 element into the intron of cloned gene was found. The transposition of the IS1 element was remarkably rapid and is likely to be sequence-specific. The use of E. coli strains that lower the copy number of vector or avoiding the presence of the problematic sequence is a solution to the inadvertent transposition of IS1. The transposition of IS1 is rare but it can occur and might confound functional studies of a plant gene.

KW - cloning

KW - transgene inactivation

KW - arabidopsis thaliana

KW - klonen

M3 - Article

VL - 22

SP - 869

EP - 871

JO - Transgenic research

JF - Transgenic research

SN - 0962-8819

IS - 4

ER -

Nap JP, Rogowski KJ, Folta A, Bargsten JW, Mlynarova L. Unexpectedly rapid IS1 transposition into an Arabidopsis chromatin remodeling gene. Transgenic research. 2013 Aug;22(4):869-871.