TY - JOUR
T1 - Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress
AU - Faber, Folkert
AU - Egli, Thomas
AU - Harder, Wim
PY - 1993/9/1
Y1 - 1993/9/1
N2 - The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.
AB - The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.
KW - escherichia coli K12
KW - stress proteine
KW - escherichia coli K12
KW - environmental stress
KW - two-dimensional electrophoresis
KW - stress protein
U2 - 10.1111/j.1574-6968.1993.tb06384.x
DO - 10.1111/j.1574-6968.1993.tb06384.x
M3 - Article
VL - 111
SP - 189
EP - 194
JO - FEMS microbiology letters
JF - FEMS microbiology letters
SN - 0378-1097
IS - 2-3
ER -