Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress

Folkert Faber; Thomas Egli; Wim Harder

Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress

Folkert Faber, Thomas Egli, Wim Harder

Life Sciences & Renewable Energy

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.

Original language	English
Pages (from-to)	189-196
Journal	FEMS Microbiology Letters
Volume	111
Issue number	2-3
Publication status	Published - 1 Sep 1993

Keywords

escherichia coli K12
environmental stress
two-dimensional electrophoresis
stress protein

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title = "Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress",

abstract = "The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.",

keywords = "escherichia coli K12, stress proteine, escherichia coli K12, environmental stress, two-dimensional electrophoresis, stress protein",

author = "Folkert Faber and Thomas Egli and Wim Harder",

year = "1993",

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language = "English",

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journal = "FEMS Microbiology Letters",

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TY - JOUR

T1 - Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia K12 as a response to pollutant stress

AU - Faber, Folkert

AU - Egli, Thomas

AU - Harder, Wim

PY - 1993/9/1

Y1 - 1993/9/1

N2 - The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.

AB - The synthesis of total cellular proteins in Escherichia coli K12 was studied in batch culture following exposure of cells to low concentrations of monochlorophenol, pentachlorophenol and cadmium chloride. Changes in protein patterns were identified after pulse-chase labelling of proteins with [35S]methionine and subsequent two-dimensional gel electrophoresis (2D-PAGE). We demonstrated that besides the induction of some stress proteins, also a transient decrease in the rate of synthesis of other proteins occurred. Two of these proteins were identified as OmpF and aspartate transcarbamoylase (ATCase). Their transient repression appeared to be a general response to stress elicited by different pollutants and may therefore be used as a general and sensitive early warning system for pollutant stress.

KW - escherichia coli K12

KW - stress proteine

KW - escherichia coli K12

KW - environmental stress

KW - two-dimensional electrophoresis

KW - stress protein

M3 - Article

VL - 111

SP - 189

EP - 196

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2-3

ER -