The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat

Alina Kunnen, M.G. van Pampus, Cees van der Schans, F. Abbas, M.M. Faas, Jan G Aarnoudse

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

OBJECTIVE:
Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.
METHODS:
Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.
RESULTS:
Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.
CONCLUSION:
Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.
Original languageEnglish
Pages (from-to)591-601
JournalOral diseases
Volume19
Issue number6
Publication statusPublished - 2013

Fingerprint

Porphyromonas gingivalis
Fetal Resorption
Lipopolysaccharides
Fetal Weight
Pregnancy
Pregnancy Complications
Periodontitis
Mothers
Blood Pressure
Proteinuria
Inflammation
Pregnancy Outcome
Fetal Development
Leukocyte Count
Placenta
Albumins
Arterial Pressure
Hypertension
Kidney
Weights and Measures

Keywords

  • periodontitis
  • pregnancy complications
  • hypertension
  • rats

Cite this

Kunnen, A., van Pampus, M. G., van der Schans, C., Abbas, F., Faas, M. M., & Aarnoudse, J. G. (2013). The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. Oral diseases, 19(6), 591-601.
Kunnen, Alina ; van Pampus, M.G. ; van der Schans, Cees ; Abbas, F. ; Faas, M.M. ; Aarnoudse, Jan G. / The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. In: Oral diseases. 2013 ; Vol. 19, No. 6. pp. 591-601.
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abstract = "OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.",
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Kunnen, A, van Pampus, MG, van der Schans, C, Abbas, F, Faas, MM & Aarnoudse, JG 2013, 'The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat', Oral diseases, vol. 19, no. 6, pp. 591-601.

The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. / Kunnen, Alina; van Pampus, M.G.; van der Schans, Cees; Abbas, F.; Faas, M.M.; Aarnoudse, Jan G.

In: Oral diseases, Vol. 19, No. 6, 2013, p. 591-601.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat

AU - Kunnen, Alina

AU - van Pampus, M.G.

AU - van der Schans, Cees

AU - Abbas, F.

AU - Faas, M.M.

AU - Aarnoudse, Jan G

PY - 2013

Y1 - 2013

N2 - OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.

AB - OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.

KW - zwangerschapsproblemen

KW - ratten

KW - periodontitis

KW - pregnancy complications

KW - hypertension

KW - rats

M3 - Article

VL - 19

SP - 591

EP - 601

JO - Oral diseases

JF - Oral diseases

SN - 1601-0825

IS - 6

ER -

Kunnen A, van Pampus MG, van der Schans C, Abbas F, Faas MM, Aarnoudse JG. The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. Oral diseases. 2013;19(6):591-601.