The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat

Alina Kunnen, M.G. van Pampus, Cees van der Schans, F. Abbas, M.M. Faas, Jan G Aarnoudse

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

OBJECTIVE:
Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.
METHODS:
Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.
RESULTS:
Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.
CONCLUSION:
Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.
Original languageEnglish
Pages (from-to)591-601
JournalOral diseases
Volume19
Issue number6
Publication statusPublished - 2013

Keywords

  • periodontitis
  • pregnancy complications
  • hypertension
  • rats

Cite this

Kunnen, A., van Pampus, M. G., van der Schans, C., Abbas, F., Faas, M. M., & Aarnoudse, J. G. (2013). The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. Oral diseases, 19(6), 591-601.
Kunnen, Alina ; van Pampus, M.G. ; van der Schans, Cees ; Abbas, F. ; Faas, M.M. ; Aarnoudse, Jan G. / The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. In: Oral diseases. 2013 ; Vol. 19, No. 6. pp. 591-601.
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abstract = "OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.",
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Kunnen, A, van Pampus, MG, van der Schans, C, Abbas, F, Faas, MM & Aarnoudse, JG 2013, 'The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat' Oral diseases, vol. 19, no. 6, pp. 591-601.

The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. / Kunnen, Alina; van Pampus, M.G.; van der Schans, Cees; Abbas, F.; Faas, M.M.; Aarnoudse, Jan G.

In: Oral diseases, Vol. 19, No. 6, 2013, p. 591-601.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat

AU - Kunnen, Alina

AU - van Pampus, M.G.

AU - van der Schans, Cees

AU - Abbas, F.

AU - Faas, M.M.

AU - Aarnoudse, Jan G

PY - 2013

Y1 - 2013

N2 - OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.

AB - OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg-1 bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg-1 bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.

KW - zwangerschapsproblemen

KW - ratten

KW - periodontitis

KW - pregnancy complications

KW - hypertension

KW - rats

M3 - Article

VL - 19

SP - 591

EP - 601

JO - Oral diseases

JF - Oral diseases

SN - 1601-0825

IS - 6

ER -

Kunnen A, van Pampus MG, van der Schans C, Abbas F, Faas MM, Aarnoudse JG. The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the rat. Oral diseases. 2013;19(6):591-601.