Structural surface changes and inflammatory responses against alginate-based microcapsules after exposure to human peritoneal fluid

Bart J de Haan, Alessandra Rossi, Marijke M Faas, Maaike J Smelt, Fabio Sonvico, Paolo Colombo, Paul de Vos

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Microencapsulation of cells is a promising approach to prevent rejection in the absence of immunosuppression. Clinical application, however, is hampered by insufficient insight in factors influencing biocompatibility of the capsules in humans. In the present study we exposed alginate-based capsules prepared of different types of alginate to human peritoneal fluid. Subsequently we studied the physicochemical changes of the capsule's surface by applying micro-Fourier Transform Infrared Spectroscopy. We did test alginate-beads and alginate-poly-L-lysine capsules prepared of different types of alginate. In all tested capsule formulations we found adsorption of components from human peritoneal fluid and clear physicochemical changes of the surface. These changes were alginate-dependent. The adsorption had no significant effects on the permselective properties of the capsule but we found a strong increase of TNFα production by human peripheral blood mononuclear cells when exposed to alginate-beads treated with human peritoneal fluid. This elevated responsiveness was not observed with alginate-PLL capsules. The results show that alginate-based capsule surfaces always undergo physicochemical changes of the surface when exposed to human peritoneal fluid. This adsorption may lead to enhancement of the inflammatory responses against the microcapsules. Our result implicate that biocompatibility measurements should not only been done with freshly prepared capsules but also with capsules that have been exposed to fluid from the implantation site in order to predict the in vivo responses. Copyright © 2011 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)394-403
Number of pages10
JournalJournal of biomedical materials research. Part A
Volume98
Issue number3
DOIs
Publication statusPublished - 1 Sep 2011

Fingerprint

Alginate
Capsules
Fluids
Biocompatibility
Adsorption
Microencapsulation
alginic acid
Phase locked loops
Fourier transform infrared spectroscopy
Blood

Keywords

  • Adsorption
  • Alginates
  • Ascitic Fluid/immunology
  • Capsules
  • Glucuronic Acid/immunology
  • Hexuronic Acids/immunology
  • Humans
  • Leukocytes, Mononuclear/immunology
  • Polylysine/analogs & derivatives
  • Tumor Necrosis Factor-alpha/immunology

Cite this

de Haan, Bart J ; Rossi, Alessandra ; Faas, Marijke M ; Smelt, Maaike J ; Sonvico, Fabio ; Colombo, Paolo ; de Vos, Paul. / Structural surface changes and inflammatory responses against alginate-based microcapsules after exposure to human peritoneal fluid. In: Journal of biomedical materials research. Part A. 2011 ; Vol. 98, No. 3. pp. 394-403.
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Structural surface changes and inflammatory responses against alginate-based microcapsules after exposure to human peritoneal fluid. / de Haan, Bart J; Rossi, Alessandra; Faas, Marijke M; Smelt, Maaike J; Sonvico, Fabio; Colombo, Paolo; de Vos, Paul.

In: Journal of biomedical materials research. Part A, Vol. 98, No. 3, 01.09.2011, p. 394-403.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - de Haan, Bart J

AU - Rossi, Alessandra

AU - Faas, Marijke M

AU - Smelt, Maaike J

AU - Sonvico, Fabio

AU - Colombo, Paolo

AU - de Vos, Paul

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AB - Microencapsulation of cells is a promising approach to prevent rejection in the absence of immunosuppression. Clinical application, however, is hampered by insufficient insight in factors influencing biocompatibility of the capsules in humans. In the present study we exposed alginate-based capsules prepared of different types of alginate to human peritoneal fluid. Subsequently we studied the physicochemical changes of the capsule's surface by applying micro-Fourier Transform Infrared Spectroscopy. We did test alginate-beads and alginate-poly-L-lysine capsules prepared of different types of alginate. In all tested capsule formulations we found adsorption of components from human peritoneal fluid and clear physicochemical changes of the surface. These changes were alginate-dependent. The adsorption had no significant effects on the permselective properties of the capsule but we found a strong increase of TNFα production by human peripheral blood mononuclear cells when exposed to alginate-beads treated with human peritoneal fluid. This elevated responsiveness was not observed with alginate-PLL capsules. The results show that alginate-based capsule surfaces always undergo physicochemical changes of the surface when exposed to human peritoneal fluid. This adsorption may lead to enhancement of the inflammatory responses against the microcapsules. Our result implicate that biocompatibility measurements should not only been done with freshly prepared capsules but also with capsules that have been exposed to fluid from the implantation site in order to predict the in vivo responses. Copyright © 2011 Wiley Periodicals, Inc.

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KW - Ascitic Fluid/immunology

KW - Capsules

KW - Glucuronic Acid/immunology

KW - Hexuronic Acids/immunology

KW - Humans

KW - Leukocytes, Mononuclear/immunology

KW - Polylysine/analogs & derivatives

KW - Tumor Necrosis Factor-alpha/immunology

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DO - 10.1002/jbm.a.33123

M3 - Article

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VL - 98

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JO - Journal of biomedical materials research. Part A

JF - Journal of biomedical materials research. Part A

SN - 1549-3296

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