Shifted T-cell polarisation after agricultural dust exposure in mice and men.

Patricia Robbe, EAJ Spierenburg, Christina Draijer, Corry Anke Brandsma, Eef Telenga, AJM van Oosterhout, Maarten Van den Berge, Marjan Luinge, Barbro Melgert, D Heederik, Wim Timens, IM Wouters, Machteld Hylkema

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

RATIONALE:
A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.
AIM:
We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.
METHODS:
DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.
RESULTS:
DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.
CONCLUSION:
Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.
Original languageEnglish
Pages (from-to)630-637
JournalThorax
Volume69
Issue number7
DOIs
Publication statusPublished - 17 Feb 2014
Externally publishedYes

Keywords

  • allergic lung disease
  • asthma
  • dust

Cite this

Robbe, P., Spierenburg, EAJ., Draijer, C., Brandsma, C. A., Telenga, E., van Oosterhout, AJM., ... Hylkema, M. (2014). Shifted T-cell polarisation after agricultural dust exposure in mice and men. Thorax, 69(7), 630-637. https://doi.org/10.1136/thoraxjnl-2013-204295
Robbe, Patricia ; Spierenburg, EAJ ; Draijer, Christina ; Brandsma, Corry Anke ; Telenga, Eef ; van Oosterhout, AJM ; Van den Berge, Maarten ; Luinge, Marjan ; Melgert, Barbro ; Heederik, D ; Timens, Wim ; Wouters, IM ; Hylkema, Machteld . / Shifted T-cell polarisation after agricultural dust exposure in mice and men. In: Thorax. 2014 ; Vol. 69, No. 7. pp. 630-637.
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title = "Shifted T-cell polarisation after agricultural dust exposure in mice and men.",
abstract = "RATIONALE:A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.AIM:We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.METHODS:DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.RESULTS:DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.CONCLUSION:Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.",
keywords = "allergic lung disease, asthma, dust, longen, astma, stof",
author = "Patricia Robbe and EAJ Spierenburg and Christina Draijer and Brandsma, {Corry Anke} and Eef Telenga and {van Oosterhout}, AJM and {Van den Berge}, Maarten and Marjan Luinge and Barbro Melgert and D Heederik and Wim Timens and IM Wouters and Machteld Hylkema",
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Robbe, P, Spierenburg, EAJ, Draijer, C, Brandsma, CA, Telenga, E, van Oosterhout, AJM, Van den Berge, M, Luinge, M, Melgert, B, Heederik, D, Timens, W, Wouters, IM & Hylkema, M 2014, 'Shifted T-cell polarisation after agricultural dust exposure in mice and men.' Thorax, vol. 69, no. 7, pp. 630-637. https://doi.org/10.1136/thoraxjnl-2013-204295

Shifted T-cell polarisation after agricultural dust exposure in mice and men. / Robbe, Patricia; Spierenburg, EAJ; Draijer, Christina; Brandsma, Corry Anke; Telenga, Eef; van Oosterhout, AJM; Van den Berge, Maarten; Luinge, Marjan; Melgert, Barbro; Heederik, D; Timens, Wim ; Wouters, IM; Hylkema, Machteld .

In: Thorax, Vol. 69, No. 7, 17.02.2014, p. 630-637.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Shifted T-cell polarisation after agricultural dust exposure in mice and men.

AU - Robbe, Patricia

AU - Spierenburg, EAJ

AU - Draijer, Christina

AU - Brandsma, Corry Anke

AU - Telenga, Eef

AU - van Oosterhout, AJM

AU - Van den Berge, Maarten

AU - Luinge, Marjan

AU - Melgert, Barbro

AU - Heederik, D

AU - Timens, Wim

AU - Wouters, IM

AU - Hylkema, Machteld

PY - 2014/2/17

Y1 - 2014/2/17

N2 - RATIONALE:A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.AIM:We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.METHODS:DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.RESULTS:DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.CONCLUSION:Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.

AB - RATIONALE:A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.AIM:We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.METHODS:DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.RESULTS:DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.CONCLUSION:Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.

KW - allergic lung disease

KW - asthma

KW - dust

KW - longen

KW - astma

KW - stof

U2 - 10.1136/thoraxjnl-2013-204295

DO - 10.1136/thoraxjnl-2013-204295

M3 - Article

VL - 69

SP - 630

EP - 637

JO - Thorax

JF - Thorax

SN - 0040-6376

IS - 7

ER -

Robbe P, Spierenburg EAJ, Draijer C, Brandsma CA, Telenga E, van Oosterhout AJM et al. Shifted T-cell polarisation after agricultural dust exposure in mice and men. Thorax. 2014 Feb 17;69(7):630-637. https://doi.org/10.1136/thoraxjnl-2013-204295