Sexual maturation protects against development of lung inflammation through estrogen

Christina Draijer, Patricia Robbe, Machteld N. Hylkema, Carian E. Boorsma, Pieter A. Klok, Wim Tiemens, Dirkje S. Postma, Catherine M. Greene, Barbro N. Melgert

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Increasing levels of estrogen and progesterone are suggested to play a role in the gender switch in asthma prevalence during puberty. We investigated whether the process of sexual maturation in mice affects the development of lung inflammation in adulthood and the contributing roles of estrogen and progesterone during this process. By inducing ovalbumin-induced lung inflammation in sexually mature and immature (ovariectomized before sexual maturation) adult mice, we showed that sexually immature adult mice developed more eosinophilic lung inflammation. This protective effect of "puberty" appears to be dependent on estrogen, as estrogen supplementation at the time of ovariectomy protected against development of lung inflammation in adulthood whereas progesterone supplementation did not. Investigating the underlying mechanism of estrogen-mediated protection, we found that estrogen-treated mice had higher expression of the anti-inflammatory mediator secretory leukoprotease inhibitor (SLPI) and lower expression of the proasthmatic cytokine IL-33 in parenchymal lung tissue and that their expressions colocalized with type II alveolar epithelial cells (AECII). Treating AECII directly with SLPI significantly inhibited IL-33 production upon stimulation with ATP. Our data suggest that estrogen during puberty has a protective effect on asthma development, which is accompanied by induction of anti-inflammatory SLPI production and inhibition of proinflammatory IL-33 production by AECII.
Original languageEnglish
Pages (from-to)166-174
JournalAmerican journal of lung cellular and molecular physiology
Volume310
Issue number2
DOIs
Publication statusPublished - 15 Jan 2016
Externally publishedYes

Keywords

  • allergic lung diseases
  • sex differences

Cite this

Draijer, Christina ; Robbe, Patricia ; Hylkema, Machteld N. ; Boorsma, Carian E. ; Klok, Pieter A. ; Tiemens, Wim ; Postma, Dirkje S. ; Greene, Catherine M. ; Melgert, Barbro N. / Sexual maturation protects against development of lung inflammation through estrogen. In: American journal of lung cellular and molecular physiology. 2016 ; Vol. 310, No. 2. pp. 166-174.
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abstract = "Increasing levels of estrogen and progesterone are suggested to play a role in the gender switch in asthma prevalence during puberty. We investigated whether the process of sexual maturation in mice affects the development of lung inflammation in adulthood and the contributing roles of estrogen and progesterone during this process. By inducing ovalbumin-induced lung inflammation in sexually mature and immature (ovariectomized before sexual maturation) adult mice, we showed that sexually immature adult mice developed more eosinophilic lung inflammation. This protective effect of {"}puberty{"} appears to be dependent on estrogen, as estrogen supplementation at the time of ovariectomy protected against development of lung inflammation in adulthood whereas progesterone supplementation did not. Investigating the underlying mechanism of estrogen-mediated protection, we found that estrogen-treated mice had higher expression of the anti-inflammatory mediator secretory leukoprotease inhibitor (SLPI) and lower expression of the proasthmatic cytokine IL-33 in parenchymal lung tissue and that their expressions colocalized with type II alveolar epithelial cells (AECII). Treating AECII directly with SLPI significantly inhibited IL-33 production upon stimulation with ATP. Our data suggest that estrogen during puberty has a protective effect on asthma development, which is accompanied by induction of anti-inflammatory SLPI production and inhibition of proinflammatory IL-33 production by AECII.",
keywords = "allergic lung diseases, sex differences, longziekten, sekseverschillen",
author = "Christina Draijer and Patricia Robbe and Hylkema, {Machteld N.} and Boorsma, {Carian E.} and Klok, {Pieter A.} and Wim Tiemens and Postma, {Dirkje S.} and Greene, {Catherine M.} and Melgert, {Barbro N.}",
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Draijer, C, Robbe, P, Hylkema, MN, Boorsma, CE, Klok, PA, Tiemens, W, Postma, DS, Greene, CM & Melgert, BN 2016, 'Sexual maturation protects against development of lung inflammation through estrogen' American journal of lung cellular and molecular physiology, vol. 310, no. 2, pp. 166-174. https://doi.org/10.1152/ajplung.00119.2015

Sexual maturation protects against development of lung inflammation through estrogen. / Draijer, Christina; Robbe, Patricia; Hylkema, Machteld N.; Boorsma, Carian E.; Klok, Pieter A.; Tiemens, Wim; Postma, Dirkje S.; Greene, Catherine M.; Melgert, Barbro N.

In: American journal of lung cellular and molecular physiology, Vol. 310, No. 2, 15.01.2016, p. 166-174.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Draijer, Christina

AU - Robbe, Patricia

AU - Hylkema, Machteld N.

AU - Boorsma, Carian E.

AU - Klok, Pieter A.

AU - Tiemens, Wim

AU - Postma, Dirkje S.

AU - Greene, Catherine M.

AU - Melgert, Barbro N.

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N2 - Increasing levels of estrogen and progesterone are suggested to play a role in the gender switch in asthma prevalence during puberty. We investigated whether the process of sexual maturation in mice affects the development of lung inflammation in adulthood and the contributing roles of estrogen and progesterone during this process. By inducing ovalbumin-induced lung inflammation in sexually mature and immature (ovariectomized before sexual maturation) adult mice, we showed that sexually immature adult mice developed more eosinophilic lung inflammation. This protective effect of "puberty" appears to be dependent on estrogen, as estrogen supplementation at the time of ovariectomy protected against development of lung inflammation in adulthood whereas progesterone supplementation did not. Investigating the underlying mechanism of estrogen-mediated protection, we found that estrogen-treated mice had higher expression of the anti-inflammatory mediator secretory leukoprotease inhibitor (SLPI) and lower expression of the proasthmatic cytokine IL-33 in parenchymal lung tissue and that their expressions colocalized with type II alveolar epithelial cells (AECII). Treating AECII directly with SLPI significantly inhibited IL-33 production upon stimulation with ATP. Our data suggest that estrogen during puberty has a protective effect on asthma development, which is accompanied by induction of anti-inflammatory SLPI production and inhibition of proinflammatory IL-33 production by AECII.

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KW - sex differences

KW - longziekten

KW - sekseverschillen

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