Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes

Hendrika A.C.F. Leeggangers, Adam Folta, Aleksandra Muras, Jan Peter Nap, Ludmila Mlynarova

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over-expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30% relative to the wild-type seeds. On the other hand, single loss-of-function mutations of the two genes did not affect seed germination. The reduction of germination in over-expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over-expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild-type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.
Original languageEnglish
Pages (from-to)318-326
JournalPsysiologia plantarum
Volume153
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • seed germination
  • arabidopsis thaliana
  • mutations
  • chromatin modifiers

Cite this

Leeggangers, Hendrika A.C.F. ; Folta, Adam ; Muras, Aleksandra ; Nap, Jan Peter ; Mlynarova, Ludmila. / Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes. In: Psysiologia plantarum. 2015 ; Vol. 153, No. 2. pp. 318-326.
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abstract = "In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over-expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30{\%} relative to the wild-type seeds. On the other hand, single loss-of-function mutations of the two genes did not affect seed germination. The reduction of germination in over-expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over-expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild-type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.",
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Leeggangers, HACF, Folta, A, Muras, A, Nap, JP & Mlynarova, L 2015, 'Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes' Psysiologia plantarum, vol. 153, no. 2, pp. 318-326. https://doi.org/10.1111/ppl.12231

Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes. / Leeggangers, Hendrika A.C.F.; Folta, Adam; Muras, Aleksandra; Nap, Jan Peter; Mlynarova, Ludmila.

In: Psysiologia plantarum, Vol. 153, No. 2, 2015, p. 318-326.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes

AU - Leeggangers, Hendrika A.C.F.

AU - Folta, Adam

AU - Muras, Aleksandra

AU - Nap, Jan Peter

AU - Mlynarova, Ludmila

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Y1 - 2015

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