Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes

Hendrika A.C.F. Leeggangers, Adam Folta, Aleksandra Muras, Jan Peter Nap, Ludmila Mlynarova

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over-expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30% relative to the wild-type seeds. On the other hand, single loss-of-function mutations of the two genes did not affect seed germination. The reduction of germination in over-expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over-expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild-type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.
Original languageEnglish
Pages (from-to)318-326
JournalPsysiologia plantarum
Volume153
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Germination
Arabidopsis
adenosinetriphosphatase
Adenosine Triphosphatases
Seeds
seed germination
seeds
Genes
germination
seed maturation
genes
chromatin
modifiers (genes)
epigenetics
Angiospermae
Stored Messenger RNA
Arabidopsis thaliana
salts
Chromatin Assembly and Disassembly
gene expression

Keywords

  • seed germination
  • arabidopsis thaliana
  • mutations
  • chromatin modifiers

Cite this

Leeggangers, Hendrika A.C.F. ; Folta, Adam ; Muras, Aleksandra ; Nap, Jan Peter ; Mlynarova, Ludmila. / Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes. In: Psysiologia plantarum. 2015 ; Vol. 153, No. 2. pp. 318-326.
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abstract = "In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over-expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30{\%} relative to the wild-type seeds. On the other hand, single loss-of-function mutations of the two genes did not affect seed germination. The reduction of germination in over-expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over-expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild-type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.",
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Leeggangers, HACF, Folta, A, Muras, A, Nap, JP & Mlynarova, L 2015, 'Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes', Psysiologia plantarum, vol. 153, no. 2, pp. 318-326. https://doi.org/10.1111/ppl.12231

Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes. / Leeggangers, Hendrika A.C.F.; Folta, Adam; Muras, Aleksandra; Nap, Jan Peter; Mlynarova, Ludmila.

In: Psysiologia plantarum, Vol. 153, No. 2, 2015, p. 318-326.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes

AU - Leeggangers, Hendrika A.C.F.

AU - Folta, Adam

AU - Muras, Aleksandra

AU - Nap, Jan Peter

AU - Mlynarova, Ludmila

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