Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain

Dirk-Jan Wijbenga, Gerrit Beldman, Anko Veen, Doede Binnema

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A bacterium belonging to the Bacillus firmus/lentus-complex and capable of growth on native potato starch was isolated from sludge of a pilot plant unit for potato-starch production. Utilization of a crude enzyme preparation obtained from the culture fluid after growth of the microorganism on native starch, resulted in complete degradation of native starch granules from potato, maize and wheat at a temperature of 37°C. Glucose was found as a major product. Production of maltose, maltotriose and maltotetraose was also observed. Native-starch-degrading activity (NSDA) could be selectively adsorbed on potato-starch granules, whereas soluble-starch-degrading activity (SSDA) remained mainly in solution. The use of such a starch-adsorbed enzyme preparation on native starch resulted in a completely changed product pattern. An increase in oligosaccharides concomitant with less glucose formation was observed. An increased conversion of soluble starch to maltopentaose was possible with this starch-adsorbed enzyme preparation. It is concluded that NSDA comes from α-amylase(s) and SSDA from glucoamylase(s) and/or α-glucosidase(s). Cultivation of B. firmus/lentus on glucose, maltose, or soluble starch resulted in substantially smaller quantities of (native) starch-degrading activity.
Original languageEnglish
Pages (from-to)180-184
JournalApplied microbiology and biotechnology
Volume35
Issue number2
DOIs
Publication statusPublished - 1991

Keywords

  • starch

Cite this

Wijbenga, Dirk-Jan ; Beldman, Gerrit ; Veen, Anko ; Binnema, Doede. / Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain. In: Applied microbiology and biotechnology. 1991 ; Vol. 35, No. 2. pp. 180-184.
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abstract = "A bacterium belonging to the Bacillus firmus/lentus-complex and capable of growth on native potato starch was isolated from sludge of a pilot plant unit for potato-starch production. Utilization of a crude enzyme preparation obtained from the culture fluid after growth of the microorganism on native starch, resulted in complete degradation of native starch granules from potato, maize and wheat at a temperature of 37°C. Glucose was found as a major product. Production of maltose, maltotriose and maltotetraose was also observed. Native-starch-degrading activity (NSDA) could be selectively adsorbed on potato-starch granules, whereas soluble-starch-degrading activity (SSDA) remained mainly in solution. The use of such a starch-adsorbed enzyme preparation on native starch resulted in a completely changed product pattern. An increase in oligosaccharides concomitant with less glucose formation was observed. An increased conversion of soluble starch to maltopentaose was possible with this starch-adsorbed enzyme preparation. It is concluded that NSDA comes from α-amylase(s) and SSDA from glucoamylase(s) and/or α-glucosidase(s). Cultivation of B. firmus/lentus on glucose, maltose, or soluble starch resulted in substantially smaller quantities of (native) starch-degrading activity.",
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Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain. / Wijbenga, Dirk-Jan; Beldman, Gerrit; Veen, Anko; Binnema, Doede.

In: Applied microbiology and biotechnology, Vol. 35, No. 2, 1991, p. 180-184.

Research output: Contribution to journalArticleAcademicpeer-review

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