Polyhydroxyalkanoate (PHA) production by thermophilic Caldimonas thermodepolymerans comb. nov. from xylan

Wen Zhou; Salvador Bertran Llorens; Peter Deuss; Gerrit Jan Willem Euverink; Janneke Krooneman

doi:10.1039/d5su00040h

Polyhydroxyalkanoate (PHA) production by thermophilic Caldimonas thermodepolymerans comb. nov. from xylan

Wen Zhou, Salvador Bertran Llorens, Peter Deuss, Gerrit Jan Willem Euverink, Janneke Krooneman

Bioconversion and fermentation technology

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

For the future circular economy, renewable carbon feedstocks manifest considerable promise for synthesizing sustainable and biodegradable polyhydroxyalkanoate (PHA). In this study, 16 wt% and 30 wt% PHA (cell dry weight) are respectively produced by thermophilic Caldimonas thermodepolymerans from beechwood xylan and wheat arabinoxylan as the sole carbon source. Moreover, an in silico study of the potential xylan-degrading proteins was conducted using proteome sequencing and CAZyme specialized bioinformatic tools. This study demonstrates the feasibility of utilizing complex polysaccharide substrates for PHA biosynthesis, thereby potentially eliminate additional processing steps and reducing overall production costs for sustainable plastic.

Original language	Dutch
Article number	2025
Pages (from-to)	1685-1690
Number of pages	6
Journal	RSC Sustainability
Volume	3
Issue number	4
DOIs	https://doi.org/10.1039/d5su00040h
Publication status	Published - 26 Feb 2025

Keywords

circular economy
biosynthese

Research Focus Areas Hanze University of Applied Sciences * (mandatory by Hanze)

Entrepreneurship

Research Focus Areas Research Centre or Centre of Expertise * (mandatory by Hanze)

Life sciences & renewable energy
Biobased chemistry

Publinova themes

Other
Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/d5su00040hLicence: CC BY-NC

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Cite this

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abstract = "For the future circular economy, renewable carbon feedstocks manifest considerable promise for synthesizing sustainable and biodegradable polyhydroxyalkanoate (PHA). In this study, 16 wt% and 30 wt% PHA (cell dry weight) are respectively produced by thermophilic Caldimonas thermodepolymerans from beechwood xylan and wheat arabinoxylan as the sole carbon source. Moreover, an in silico study of the potential xylan-degrading proteins was conducted using proteome sequencing and CAZyme specialized bioinformatic tools. This study demonstrates the feasibility of utilizing complex polysaccharide substrates for PHA biosynthesis, thereby potentially eliminate additional processing steps and reducing overall production costs for sustainable plastic.",

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AU - Euverink, Gerrit Jan Willem

AU - Krooneman, Janneke

PY - 2025/2/26

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AB - For the future circular economy, renewable carbon feedstocks manifest considerable promise for synthesizing sustainable and biodegradable polyhydroxyalkanoate (PHA). In this study, 16 wt% and 30 wt% PHA (cell dry weight) are respectively produced by thermophilic Caldimonas thermodepolymerans from beechwood xylan and wheat arabinoxylan as the sole carbon source. Moreover, an in silico study of the potential xylan-degrading proteins was conducted using proteome sequencing and CAZyme specialized bioinformatic tools. This study demonstrates the feasibility of utilizing complex polysaccharide substrates for PHA biosynthesis, thereby potentially eliminate additional processing steps and reducing overall production costs for sustainable plastic.

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