Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Janneke Krooneman; F Faber; AC Alderkamp; SJHWO Elferink; F Driehuis; [No Value] Cleenwerck; J Swings; JC Gottschal; M Vancanneyt

doi:10.1099/00207713-52-2-639

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Janneke Krooneman, F Faber, AC Alderkamp, SJHWO Elferink, F Driehuis, [No Value] Cleenwerck, J Swings, JC Gottschal, M Vancanneyt

Biorefinery

Rijksuniversiteit Groningen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).

Original language	English
Pages (from-to)	639-646
Number of pages	8
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	52
Issue number	2
DOIs	https://doi.org/10.1099/00207713-52-2-639
Publication status	Published - 1 Mar 2002

Keywords

anaerobiosis
environmental biodegradation
dna, bacterial/chemistry
lactobacillus/classification
molecular sequence data
nucleic acid hybridization
phylogeny
propionates/analysis
propylene glycol/metabolism
rna, ribosomal, 16S/chemistry
silage/microbiology
zea mays/microbiology

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10.1099/00207713-52-2-639

1 Paper
1 Article

Anaerobic metabolic activities of Lactobacillus buchneri in maize silage: ISME-8
Krooneman, J., Oude Elferink, S. J. W. H., Driehuis, F., Spoelstra, S. F., Forney, L. J. & Gottschal, J., 1998.
Research output: Contribution to conference › Paper › Academic
Isolation of Alcaligenes sp strain L6 at low oxygen concentrations and degradation of 3-chlorobenzoate via a pathway not involving (chloro)catechols
Krooneman, J., Wieringa, E. B. A., Moore, E. R. B., Gerritse, J., Prins, R. A. & Gottschal, J. C., 1 Jul 1996, In: Applied microbiology. 62, 7, p. 2427-2434 8 p.
Research output: Contribution to journal › Article › Academic › peer-review

Open Access

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Krooneman, J., Faber, F., Alderkamp, AC., Elferink, SJHWO., Driehuis, F., Cleenwerck, N. V., Swings, J., Gottschal, JC., & Vancanneyt, M. (2002). Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. International Journal of Systematic and Evolutionary Microbiology, 52(2), 639-646. https://doi.org/10.1099/00207713-52-2-639

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title = "Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage",

abstract = "Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).",

keywords = "anaerobiosis, environmental biodegradation, dna, bacterial/chemistry, lactobacillus/classification, molecular sequence data, nucleic acid hybridization, phylogeny, propionates/analysis, propylene glycol/metabolism, rna, ribosomal, 16S/chemistry, silage/microbiology, zea mays/microbiology, microbiologie",

author = "Janneke Krooneman and F Faber and AC Alderkamp and SJHWO Elferink and F Driehuis and Cleenwerck, {[No Value]} and J Swings and JC Gottschal and M Vancanneyt",

year = "2002",

month = mar,

day = "1",

doi = "10.1099/00207713-52-2-639",

language = "English",

volume = "52",

pages = "639--646",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Society for General Microbiology",

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Krooneman, J , Faber, F, Alderkamp, AC, Elferink, SJHWO, Driehuis, F, Cleenwerck, NV, Swings, J, Gottschal, JC & Vancanneyt, M 2002, 'Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage', International Journal of Systematic and Evolutionary Microbiology, vol. 52, no. 2, pp. 639-646. https://doi.org/10.1099/00207713-52-2-639

TY - JOUR

T1 - Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

AU - Krooneman, Janneke

AU - Faber, F

AU - Alderkamp, AC

AU - Elferink, SJHWO

AU - Driehuis, F

AU - Cleenwerck, [No Value]

AU - Swings, J

AU - Gottschal, JC

AU - Vancanneyt, M

PY - 2002/3/1

Y1 - 2002/3/1

N2 - Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).

AB - Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).

KW - anaerobiosis

KW - environmental biodegradation

KW - dna, bacterial/chemistry

KW - lactobacillus/classification

KW - molecular sequence data

KW - nucleic acid hybridization

KW - phylogeny

KW - propionates/analysis

KW - propylene glycol/metabolism

KW - rna, ribosomal, 16S/chemistry

KW - silage/microbiology

KW - zea mays/microbiology

KW - microbiologie

U2 - 10.1099/00207713-52-2-639

DO - 10.1099/00207713-52-2-639

M3 - Article

C2 - 11931178

VL - 52

SP - 639

EP - 646

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 2

ER -

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Abstract

Keywords

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Research output

Anaerobic metabolic activities of Lactobacillus buchneri in maize silage: ISME-8

Isolation of Alcaligenes sp strain L6 at low oxygen concentrations and degradation of 3-chlorobenzoate via a pathway not involving (chloro)catechols

Cite this