Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

J Krooneman, F Faber, A C Alderkamp, S J H W Oude Elferink, F Driehuis, I Cleenwerck, J Swings, J C Gottschal, M Vancanneyt

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T (= DSM 14421T).

Original languageEnglish
Pages (from-to)639-646
JournalInternational journal of systematic and evolutionary microbiology
Volume52
Issue numberPt 2
DOIs
Publication statusPublished - Mar 2002

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Keywords

  • anaerobiosis
  • biodegradation, environmental
  • dna, bacterial/chemistry
  • lactobacillus/classification
  • molecular sequence data
  • nucleic acid hybridization
  • phylogeny
  • propionates/analysis
  • propylene glycol/metabolism
  • rna, ribosomal, 16S/chemistry
  • silage/microbiology
  • zea mays/microbiology

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