Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Janneke Krooneman; F Faber; AC Alderkamp; SJHWO Elferink; F Driehuis; [No Value] Cleenwerck; J Swings; JC Gottschal; M Vancanneyt

doi:10.1099/00207713-52-2-639

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Janneke Krooneman, F Faber, AC Alderkamp, SJHWO Elferink, F Driehuis, [No Value] Cleenwerck, J Swings, JC Gottschal, M Vancanneyt

Rijksuniversiteit Groningen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).

Original language	English
Pages (from-to)	639-646
Number of pages	8
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	52
Issue number	2
DOIs	https://doi.org/10.1099/00207713-52-2-639
Publication status	Published - 1 Mar 2002

Keywords

anaerobiosis
environmental biodegradation
dna, bacterial/chemistry
lactobacillus/classification
molecular sequence data
nucleic acid hybridization
phylogeny
propionates/analysis
propylene glycol/metabolism
rna, ribosomal, 16S/chemistry
silage/microbiology
zea mays/microbiology

Access to Document

10.1099/00207713-52-2-639

3 Article
1 Paper

Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans
Zhou, W., Colpa, D., Permentier, H., Offringa, R. A., Rohrbach, L., Euverink, G. J. W. & Krooneman, J., Jul 2023, In: Resources, Conservation and Recycling. 194
Research output: Contribution to journal › Article › Academic › peer-review

Open Access
Polyhydroxyalkanoates (PHAs) synthesis and degradation by microbes and applications towards a circular economy
Zhou, W., Bergsma, S., Colpa, D. I., Euverink, G.-J. W. & Krooneman, J., 1 Sept 2023, In: Journal of Environmental Management. 341, 118033
Research output: Contribution to journal › Article › Academic › peer-review

Open Access
Anaerobic metabolic activities of Lactobacillus buchneri in maize silage: ISME-8
Krooneman, J., Oude Elferink, S. J. W. H., Driehuis, F., Spoelstra, S. F., Forney, L. J. & Gottschal, J., 1998.
Research output: Contribution to conference › Paper › Academic

3 Oral presentation

Lezing kick-off Interreg project Biotech Talent Unlocked
Krooneman, J. (Speaker)
31 Mar 2023
Activity: Talk or presentation › Oral presentation
Lectorale rede Janneke Krooneman
Krooneman, J. (Speaker)
5 Apr 2023
Activity: Talk or presentation › Oral presentation
Praktijkgericht Fermentatieonderzoek: Faciliteiten, van lab tot productieschaal (Lezing Lectorenplatform Biobased Economy)
Krooneman, J. (Speaker)
15 Dec 2022
Activity: Talk or presentation › Oral presentation

Universiteit van Noorden krijgt gestalte
Krooneman, J. & Euverink, G. J. W.
1/06/23
1 item of Media coverage
Press/Media: Public Engagement Activities

Cite this

Krooneman, J., Faber, F., Alderkamp, AC., Elferink, SJHWO., Driehuis, F., Cleenwerck, N. V., Swings, J., Gottschal, JC., & Vancanneyt, M. (2002). Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. International Journal of Systematic and Evolutionary Microbiology, 52(2), 639-646. https://doi.org/10.1099/00207713-52-2-639

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title = "Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage",

abstract = "Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).",

keywords = "anaerobiosis, environmental biodegradation, dna, bacterial/chemistry, lactobacillus/classification, molecular sequence data, nucleic acid hybridization, phylogeny, propionates/analysis, propylene glycol/metabolism, rna, ribosomal, 16S/chemistry, silage/microbiology, zea mays/microbiology, microbiologie",

author = "Janneke Krooneman and F Faber and AC Alderkamp and SJHWO Elferink and F Driehuis and Cleenwerck, {[No Value]} and J Swings and JC Gottschal and M Vancanneyt",

year = "2002",

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doi = "10.1099/00207713-52-2-639",

language = "English",

volume = "52",

pages = "639--646",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Society for General Microbiology",

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Krooneman, J , Faber, F, Alderkamp, AC, Elferink, SJHWO, Driehuis, F, Cleenwerck, NV, Swings, J, Gottschal, JC & Vancanneyt, M 2002, 'Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage', International Journal of Systematic and Evolutionary Microbiology, vol. 52, no. 2, pp. 639-646. https://doi.org/10.1099/00207713-52-2-639

TY - JOUR

T1 - Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

AU - Krooneman, Janneke

AU - Faber, F

AU - Alderkamp, AC

AU - Elferink, SJHWO

AU - Driehuis, F

AU - Cleenwerck, [No Value]

AU - Swings, J

AU - Gottschal, JC

AU - Vancanneyt, M

PY - 2002/3/1

Y1 - 2002/3/1

N2 - Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).

AB - Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 × 107 c.f.u. g-1) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivorans sp. nov. is proposed. The type strain is LMG 19667T ( = DSM 14421T).

KW - anaerobiosis

KW - environmental biodegradation

KW - dna, bacterial/chemistry

KW - lactobacillus/classification

KW - molecular sequence data

KW - nucleic acid hybridization

KW - phylogeny

KW - propionates/analysis

KW - propylene glycol/metabolism

KW - rna, ribosomal, 16S/chemistry

KW - silage/microbiology

KW - zea mays/microbiology

KW - microbiologie

U2 - 10.1099/00207713-52-2-639

DO - 10.1099/00207713-52-2-639

M3 - Article

C2 - 11931178

SN - 1466-5026

VL - 52

SP - 639

EP - 646

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 2

ER -

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Abstract

Keywords

Access to Document

Fingerprint

Research output

Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Polyhydroxyalkanoates (PHAs) synthesis and degradation by microbes and applications towards a circular economy

Anaerobic metabolic activities of Lactobacillus buchneri in maize silage: ISME-8

Activities

Lezing kick-off Interreg project Biotech Talent Unlocked

Lectorale rede Janneke Krooneman

Praktijkgericht Fermentatieonderzoek: Faciliteiten, van lab tot productieschaal (Lezing Lectorenplatform Biobased Economy)

Press/Media

Universiteit van Noorden krijgt gestalte

Cite this