Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Wen Zhou; Dana Colpa; Hjalmar Permentier; Ruben Ate Offringa; Leon Rohrbach; Gert Jan Willem Euverink; Janneke Krooneman

doi:10.1016/j.resconrec.2023.107006

Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Wen Zhou, Dana Colpa, Hjalmar Permentier, Ruben Ate Offringa, Leon Rohrbach, Gert Jan Willem Euverink, Janneke Krooneman

University of Groningen; Faculty of Science and Engineering
University of Groningen, Department of Analytical Biochemistry
University of Groningen, Faculty of Science and Engineering, Engineering and Technology Institute Groningen, Green Chemical Reaction Engineering

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.

Original language	English
Journal	Resources, Conservation and Recycling
Volume	194
Early online date	24 Apr 2023
DOIs	https://doi.org/10.1016/j.resconrec.2023.107006
Publication status	Published - Jul 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 12 Responsible Consumption and Production
SDG 13 Climate Action

Keywords

bacteria
biopolymers
pha production

Access to Document

10.1016/j.resconrec.2023.107006Licence: CC BY

1 Finished
1 Active

BIOTECH TALENT UNLOCKED
Krooneman, J. (PI)
1/01/23 → 31/12/26
Project: Research
More Science than Fiction: growing spare parts ex vivo
Heeres, A. (PI), Krooneman, J. (PI) & Siebring, J. (PI)
1/04/22 → 30/04/23
Project: Research

17 Article
1 Book
1 Report
1 Chapter
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- 1 Digital or Visual Products

Hanze Impact Podcast: #6 Groene Chemie met André Heeres en Janneke Krooneman
Krooneman, J. (Performer) & Heeres, A. (Performer), 19 May 2023
Research output: Non-textual form › Digital or Visual Products › Professional
Polyhydroxyalkanoates (PHAs) synthesis and degradation by microbes and applications towards a circular economy
Zhou, W., Bergsma, S., Colpa, D. I., Euverink, G.-J. W. & Krooneman, J., 1 Sept 2023, In: Journal of Environmental Management. 341, 118033
Research output: Contribution to journal › Article › Academic › peer-review

Open Access
The impact of carbon to nitrogen ratios and pH on the microbial prevalence and polyhydroxybutyrate production levels using a mixed microbial starter culture
Zhou, W., Colpa, D., Geurkink, B., Euverink, G. J. W. & Krooneman, J., 10 Mar 2022, In: Science of the Total Environment. 811, 152341.
Research output: Contribution to journal › Article › Academic › peer-review

Open Access

Barn Talk Towards Renewable Molecules: Koppeling van de energiesector aan de chemische sector (materialentransitie)
Heeres, A. (Speaker) & Krooneman, J. (Speaker)
12 Apr 2023
Activity: Talk or presentation › Invited talk
Lezing kick-off Interreg project Biotech Talent Unlocked
Krooneman, J. (Speaker)
31 Mar 2023
Activity: Talk or presentation › Oral presentation
Klimaatexpeditie naar Spitsbergen
Krooneman, J. (Speaker)
9 Mar 2023
Activity: Talk or presentation › Invited talk

Universiteit van Noorden krijgt gestalte
Krooneman, J. & Euverink, G. J. W.
1/06/23
1 item of Media coverage
Press/Media: Public Engagement Activities
Inauguratie Janneke Krooneman als lector Bioconversie en fermentatietechnologie
Krooneman, J.
19/12/22
1 item of Media coverage
Press/Media: Expert Comment
Project Circulaire Biopolymeren Waardeketens krijgt vervolg: "We hebben nu beter grip of de biologie"
Krooneman, J.
31/08/22
1 item of Media coverage
Press/Media: Expert Comment

Cite this

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title = "Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans",

abstract = "Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt\% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.",

keywords = "PHA productie, bacteri{\"e}n, biopolymeren, bacteria, biopolymers, pha production",

author = "Wen Zhou and Dana Colpa and Hjalmar Permentier and Offringa, \{Ruben Ate\} and Leon Rohrbach and Euverink, \{Gert Jan Willem\} and Janneke Krooneman",

year = "2023",

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doi = "10.1016/j.resconrec.2023.107006",

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T1 - Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

AU - Zhou, Wen

AU - Colpa, Dana

AU - Permentier, Hjalmar

AU - Offringa, Ruben Ate

AU - Rohrbach, Leon

AU - Euverink, Gert Jan Willem

AU - Krooneman, Janneke

PY - 2023/7

Y1 - 2023/7

N2 - Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.

AB - Accumulation of non-degradable plastic waste in the environment might be prevented by the use of biodegradable polyhydroxyalkanoate (PHA). In this study, the thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest PHA granules were found in the cells within 48 h using 20 g/L xylose, a C/N ratio of 100, an initial pH of 7, at 50 °C. The substrate consumption, pH changes, and cell growth were monitored, revealing the time dependency of PHA production in S. thermodepolymerans. The metabolic pathways from xylose to PHA were identified based on proteomic analysis, revealing involvement of classic phaCAB, de novo fatty acid biosynthesis, and fatty acid β-oxidation. In addition, it was shown that S. thermodepolymerans degraded extracellular PHA with a high efficiency at 50 °C.

KW - PHA productie

KW - bacteriën

KW - biopolymeren

KW - bacteria

KW - biopolymers

KW - pha production

U2 - 10.1016/j.resconrec.2023.107006

DO - 10.1016/j.resconrec.2023.107006

M3 - Article

SN - 0921-3449

VL - 194

JO - Resources, Conservation and Recycling

JF - Resources, Conservation and Recycling

ER -

Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Abstract

UN SDGs

Keywords

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