Identification of two types of protein immunochemically related to urinary urokinase occurring in human plasma

D J Binnema, G Dooijewaard, J J van Iersel

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Plasma urokinase, a plasminogen activator immunochemically related to urinary urokinase (UK), was removed from human plasma (3.5 ng/ml) by immuno-depletion with antibodies raised against UK. The remaining plasminogen activator activity of the depleted plasma could not be inhibited by anti-UK antibodies and a sensitive ELISA for UK did not detect any UK levels that were higher than the background of the assay (0.1 ng/ml). However, when the depleted plasma was subjected to SDS-PAGE, substantial amounts of protein were found hereafter around 110 and 46 kD which now gave a positive reaction in the ELISA (35-350 ng/ml plasma). From these observations it is concluded that in human plasma two types of UK-related protein occur: Type I, among which the plasma urokinase, has antigenic determinants which are directly accessible to the anti-UK antibodies, Type II has determinants in a latent form. The function of the 110 kD type-II protein is that of a plasminogen activator; that of the 46 kD protein is not yet clear.

Original languageEnglish
Pages (from-to)162-168
JournalBiochemical and biophysical research communications
Volume142
Issue number1
DOIs
Publication statusPublished - 15 Jan 1987

Fingerprint

Plasma (human)
Urokinase-Type Plasminogen Activator
Proteins
Plasminogen Activators
Plasmas
Antibodies
Anti-Idiotypic Antibodies
Enzyme-Linked Immunosorbent Assay
Epitopes
Polyacrylamide Gel Electrophoresis
Assays

Keywords

  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Activation
  • Humans
  • Molecular Weight
  • Sodium Dodecyl Sulfate
  • Tissue Plasminogen Activator
  • Urokinase-Type Plasminogen Activator
  • Research Support, Non-U.S. Gov't

Cite this

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title = "Identification of two types of protein immunochemically related to urinary urokinase occurring in human plasma",
abstract = "Plasma urokinase, a plasminogen activator immunochemically related to urinary urokinase (UK), was removed from human plasma (3.5 ng/ml) by immuno-depletion with antibodies raised against UK. The remaining plasminogen activator activity of the depleted plasma could not be inhibited by anti-UK antibodies and a sensitive ELISA for UK did not detect any UK levels that were higher than the background of the assay (0.1 ng/ml). However, when the depleted plasma was subjected to SDS-PAGE, substantial amounts of protein were found hereafter around 110 and 46 kD which now gave a positive reaction in the ELISA (35-350 ng/ml plasma). From these observations it is concluded that in human plasma two types of UK-related protein occur: Type I, among which the plasma urokinase, has antigenic determinants which are directly accessible to the anti-UK antibodies, Type II has determinants in a latent form. The function of the 110 kD type-II protein is that of a plasminogen activator; that of the 46 kD protein is not yet clear.",
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Identification of two types of protein immunochemically related to urinary urokinase occurring in human plasma. / Binnema, D J; Dooijewaard, G; van Iersel, J J.

In: Biochemical and biophysical research communications, Vol. 142, No. 1, 15.01.1987, p. 162-168.

Research output: Contribution to journalArticleAcademicpeer-review

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N2 - Plasma urokinase, a plasminogen activator immunochemically related to urinary urokinase (UK), was removed from human plasma (3.5 ng/ml) by immuno-depletion with antibodies raised against UK. The remaining plasminogen activator activity of the depleted plasma could not be inhibited by anti-UK antibodies and a sensitive ELISA for UK did not detect any UK levels that were higher than the background of the assay (0.1 ng/ml). However, when the depleted plasma was subjected to SDS-PAGE, substantial amounts of protein were found hereafter around 110 and 46 kD which now gave a positive reaction in the ELISA (35-350 ng/ml plasma). From these observations it is concluded that in human plasma two types of UK-related protein occur: Type I, among which the plasma urokinase, has antigenic determinants which are directly accessible to the anti-UK antibodies, Type II has determinants in a latent form. The function of the 110 kD type-II protein is that of a plasminogen activator; that of the 46 kD protein is not yet clear.

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