Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A

Fean D Sarian, Rachel M van der Kaaij, Slavko Kralj, Dirk-Jan Wijbenga, Doede J. Binnema, Marc J E C van der Maarel, Lubbert Dijkhuizen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.

Original languageEnglish
Pages (from-to)645-654
JournalApplied microbiology and biotechnology
Volume93
Issue number2
DOIs
Publication statusPublished - Jan 2012

Keywords

  • Actinomycetales
  • Cluster Analysis
  • DNA, Bacterial
  • DNA, Ribosomal
  • Maltose
  • Manihot
  • Molecular Sequence Data
  • Oligosaccharides
  • Phylogeny
  • RNA, Ribosomal, 16S
  • Sequence Analysis, DNA
  • Solanum tuberosum
  • Starch
  • Temperature
  • Trisaccharides
  • Triticum
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Sarian, F. D., van der Kaaij, R. M., Kralj, S., Wijbenga, D-J., Binnema, D. J., van der Maarel, M. J. E. C., & Dijkhuizen, L. (2012). Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A. Applied microbiology and biotechnology, 93(2), 645-654. https://doi.org/10.1007/s00253-011-3436-7
Sarian, Fean D ; van der Kaaij, Rachel M ; Kralj, Slavko ; Wijbenga, Dirk-Jan ; Binnema, Doede J. ; van der Maarel, Marc J E C ; Dijkhuizen, Lubbert. / Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A. In: Applied microbiology and biotechnology. 2012 ; Vol. 93, No. 2. pp. 645-654.
@article{3bb03d33ec36448388aedfcb1b997dbf,
title = "Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A",
abstract = "Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.",
keywords = "Actinomycetales, Cluster Analysis, DNA, Bacterial, DNA, Ribosomal, Maltose, Manihot, Molecular Sequence Data, Oligosaccharides, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Solanum tuberosum, Starch, Temperature, Trisaccharides, Triticum, Journal Article, Research Support, Non-U.S. Gov't",
author = "Sarian, {Fean D} and {van der Kaaij}, {Rachel M} and Slavko Kralj and Dirk-Jan Wijbenga and Binnema, {Doede J.} and {van der Maarel}, {Marc J E C} and Lubbert Dijkhuizen",
year = "2012",
month = "1",
doi = "10.1007/s00253-011-3436-7",
language = "English",
volume = "93",
pages = "645--654",
journal = "Applied microbiology and biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "2",

}

Sarian, FD, van der Kaaij, RM, Kralj, S, Wijbenga, D-J, Binnema, DJ, van der Maarel, MJEC & Dijkhuizen, L 2012, 'Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A' Applied microbiology and biotechnology, vol. 93, no. 2, pp. 645-654. https://doi.org/10.1007/s00253-011-3436-7

Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A. / Sarian, Fean D; van der Kaaij, Rachel M; Kralj, Slavko; Wijbenga, Dirk-Jan; Binnema, Doede J.; van der Maarel, Marc J E C; Dijkhuizen, Lubbert.

In: Applied microbiology and biotechnology, Vol. 93, No. 2, 01.2012, p. 645-654.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A

AU - Sarian, Fean D

AU - van der Kaaij, Rachel M

AU - Kralj, Slavko

AU - Wijbenga, Dirk-Jan

AU - Binnema, Doede J.

AU - van der Maarel, Marc J E C

AU - Dijkhuizen, Lubbert

PY - 2012/1

Y1 - 2012/1

N2 - Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.

AB - Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules.

KW - Actinomycetales

KW - Cluster Analysis

KW - DNA, Bacterial

KW - DNA, Ribosomal

KW - Maltose

KW - Manihot

KW - Molecular Sequence Data

KW - Oligosaccharides

KW - Phylogeny

KW - RNA, Ribosomal, 16S

KW - Sequence Analysis, DNA

KW - Solanum tuberosum

KW - Starch

KW - Temperature

KW - Trisaccharides

KW - Triticum

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00253-011-3436-7

DO - 10.1007/s00253-011-3436-7

M3 - Article

VL - 93

SP - 645

EP - 654

JO - Applied microbiology and biotechnology

JF - Applied microbiology and biotechnology

SN - 0175-7598

IS - 2

ER -