Diversity in robustness of Lactococcus lactis strains during heat stress, oxidative stress, and spray drying stress

Annereinou R Dijkstra, Meily C Setyawati, Jumamurat R Bayjanov, Wynand Alkema, Sacha A F T van Hijum, Peter A Bron, Jeroen Hugenholtz

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this study we tested 39 Lactococcus lactis strains isolated from diverse habitats for their robustness under heat and oxidative stress, demonstrating high diversity in survival (up to 4 log units). Strains with an L. lactis subsp. lactis phenotype generally displayed more-robust phenotypes than strains with an L. lactis subsp. cremoris phenotype, whereas the habitat from which the strains had been isolated did not appear to influence stress survival. Comparison of the stress survival phenotypes with already available comparative genomic data sets revealed that the absence or presence of specific genes, including genes encoding a GntR family transcriptional regulator, a manganese ABC transporter permease, a cellobiose phosphotransferase system (PTS) component, the FtsY protein, and hypothetical proteins, was associated with heat or oxidative stress survival. Finally, 14 selected strains also displayed diversity in survival after spray drying, ranging from 20% survival for the most robust strains, which appears acceptable for industrial application, to 0.1% survival for the least-tolerant strains. The high and low levels of survival upon spray drying correlated clearly with the combined robustness under heat and oxidative stress. These results demonstrate the relevance of screening culture collections for robustness under heat and oxidative stress on top of the typical screening for acidifying and flavor-forming properties.

Original languageEnglish
Pages (from-to)603-611
JournalApplied and environmental microbiology
Volume80
Issue number2
DOIs
Publication statusPublished - Jan 2014

Keywords

  • bacterial proteins/genetics
  • dna-binding proteins/genetics
  • genes, bacterial
  • genetic variation
  • heat-shock response
  • lactococcus lactis/isolation & purification
  • oxidative Stress/genetics
  • phenotype
  • phosphoenolpyruvate sugar phosphotransferase system/genetics
  • stress, physiological

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    Dijkstra, A. R., Setyawati, M. C., Bayjanov, J. R., Alkema, W., van Hijum, S. A. F. T., Bron, P. A., & Hugenholtz, J. (2014). Diversity in robustness of Lactococcus lactis strains during heat stress, oxidative stress, and spray drying stress. Applied and environmental microbiology, 80(2), 603-611. https://doi.org/10.1128/AEM.03434-13