Abstract
Bacillus subtilis is a Gram-positive bacterium that is frequently used in the bioindustry for the production of various proteins, because of its superior protein secretion capacities. To determine optimal conditions for protein secretion by B. subtilis, a quick and sensitive method for measuring protein secretion is crucial. A fast and universal assay is most useful for detecting diverse proteins in a high-throughput manner. In this study, we introduce a split-luciferase-based method for measuring protein secretion by B. subtilis. The NanoBiT system was used to monitor secretion of four different proteins: xylanase A, amylase M, protein glutaminase A, and GFP nanobody. Our findings underscore the split-luciferase system as a quick, sensitive, and user-friendly method.
| Original language | English |
|---|---|
| Journal | Microbiology (United Kingdom) |
| Volume | 170 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 7 Jun 2024 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 15 Life on Land
Keywords
- Amylases/metabolism
- Bacillus subtilis/metabolism
- Bacterial Proteins/metabolism
- Endo-1,4-beta Xylanases/metabolism
- Glutaminase/metabolism
- Green Fluorescent Proteins/genetics
- Luciferases/metabolism
- Protein Transport
Research Focus Areas Hanze University of Applied Sciences * (mandatory by Hanze)
- Energy
Research Focus Areas Research Centre or Centre of Expertise * (mandatory by Hanze)
- Healthcare & Biotechnology
- Life Sciences
- Transition Bio Economy
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