Определение антоцианов лепестков цветков тюльпанов способом обращенно-фазовой ВЭЖХ

Translated title of the contribution: Determination of tulip flower anthocyanins by reversed-phase HPLC

V.I. Deineka, Yaroslava Kulchenko, L.A. Deineka, A.N. Chulkov, V.F. Selemenev

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Reversed-phase HPLC with mass spectrometric and diode array detection as well as some literature data were used to reveal the individual types of solutes in anthocyanin complexes of tulip flower petals that are responsible for tulip flower petals coloration of the samples available in the local flower market. It has been found that the main components of the complexes are 3-rutinosides and their 2”’ and 3”’ acylated with acetic acid derivatives of the three anthocyanidins - delphinidin, cyanidin and pelargonidin in the color dependent ratios, though trace quantities of 3-glucosides were found in some cases. For the anthocyanin structure confirmation a correlation analysis of solute retentions of cyanidin or pelargonidin derivatives vs that of delphinidin was proposed based upon equivalence of structures alteration in the solute pairs for each series. The specificity of solutes retention modes was revealed by relative retention analysis, the trend parameters reflected particularities of chromatographic behavior as well as that of electron spectra of the solutes. The difference of acylated anthocyanins retentions was proposed to disclose the conformation states of solutes in the sorbent interface.
Original languageRussian
Article number4
Pages (from-to)464-471
JournalСорбционные и хроматографические процессы
Volume16
Issue number4
Publication statusPublished - 2016

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delphinidin
Anthocyanins
Derivatives
Glucosides
Sorbents
Acetic Acid
Conformations
Diodes
Color
Electrons
cyanidin
pelargonidin

Keywords

  • anthocyanins
  • tulips
  • reversed-phase HPLC
  • retention particularities
  • correlation analysis
  • similarities series
  • separation map

Cite this

@article{3cddb609dd4544d1976c3a2ad69609aa,
title = "Определение антоцианов лепестков цветков тюльпанов способом обращенно-фазовой ВЭЖХ",
abstract = "Reversed-phase HPLC with mass spectrometric and diode array detection as well as some literature data were used to reveal the individual types of solutes in anthocyanin complexes of tulip flower petals that are responsible for tulip flower petals coloration of the samples available in the local flower market. It has been found that the main components of the complexes are 3-rutinosides and their 2”’ and 3”’ acylated with acetic acid derivatives of the three anthocyanidins - delphinidin, cyanidin and pelargonidin in the color dependent ratios, though trace quantities of 3-glucosides were found in some cases. For the anthocyanin structure confirmation a correlation analysis of solute retentions of cyanidin or pelargonidin derivatives vs that of delphinidin was proposed based upon equivalence of structures alteration in the solute pairs for each series. The specificity of solutes retention modes was revealed by relative retention analysis, the trend parameters reflected particularities of chromatographic behavior as well as that of electron spectra of the solutes. The difference of acylated anthocyanins retentions was proposed to disclose the conformation states of solutes in the sorbent interface.",
keywords = "anthocyanine, tulips, hplc, anthocyanins, tulips, reversed-phase HPLC, retention particularities, correlation analysis, similarities series, separation map",
author = "V.I. Deineka and Yaroslava Kulchenko and L.A. Deineka and A.N. Chulkov and V.F. Selemenev",
year = "2016",
language = "Russian",
volume = "16",
pages = "464--471",
journal = "Сорбционные и хроматографические процессы",
issn = "1680-0613",
publisher = "Voronezh State University",
number = "4",

}

Определение антоцианов лепестков цветков тюльпанов способом обращенно-фазовой ВЭЖХ. / Deineka, V.I.; Kulchenko, Yaroslava; Deineka, L.A.; Chulkov, A.N.; Selemenev, V.F.

In: Сорбционные и хроматографические процессы, Vol. 16, No. 4, 4, 2016, p. 464-471.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Определение антоцианов лепестков цветков тюльпанов способом обращенно-фазовой ВЭЖХ

AU - Deineka, V.I.

AU - Kulchenko, Yaroslava

AU - Deineka, L.A.

AU - Chulkov, A.N.

AU - Selemenev, V.F.

PY - 2016

Y1 - 2016

N2 - Reversed-phase HPLC with mass spectrometric and diode array detection as well as some literature data were used to reveal the individual types of solutes in anthocyanin complexes of tulip flower petals that are responsible for tulip flower petals coloration of the samples available in the local flower market. It has been found that the main components of the complexes are 3-rutinosides and their 2”’ and 3”’ acylated with acetic acid derivatives of the three anthocyanidins - delphinidin, cyanidin and pelargonidin in the color dependent ratios, though trace quantities of 3-glucosides were found in some cases. For the anthocyanin structure confirmation a correlation analysis of solute retentions of cyanidin or pelargonidin derivatives vs that of delphinidin was proposed based upon equivalence of structures alteration in the solute pairs for each series. The specificity of solutes retention modes was revealed by relative retention analysis, the trend parameters reflected particularities of chromatographic behavior as well as that of electron spectra of the solutes. The difference of acylated anthocyanins retentions was proposed to disclose the conformation states of solutes in the sorbent interface.

AB - Reversed-phase HPLC with mass spectrometric and diode array detection as well as some literature data were used to reveal the individual types of solutes in anthocyanin complexes of tulip flower petals that are responsible for tulip flower petals coloration of the samples available in the local flower market. It has been found that the main components of the complexes are 3-rutinosides and their 2”’ and 3”’ acylated with acetic acid derivatives of the three anthocyanidins - delphinidin, cyanidin and pelargonidin in the color dependent ratios, though trace quantities of 3-glucosides were found in some cases. For the anthocyanin structure confirmation a correlation analysis of solute retentions of cyanidin or pelargonidin derivatives vs that of delphinidin was proposed based upon equivalence of structures alteration in the solute pairs for each series. The specificity of solutes retention modes was revealed by relative retention analysis, the trend parameters reflected particularities of chromatographic behavior as well as that of electron spectra of the solutes. The difference of acylated anthocyanins retentions was proposed to disclose the conformation states of solutes in the sorbent interface.

KW - anthocyanine

KW - tulips

KW - hplc

KW - anthocyanins

KW - tulips

KW - reversed-phase HPLC

KW - retention particularities

KW - correlation analysis

KW - similarities series

KW - separation map

M3 - Article

VL - 16

SP - 464

EP - 471

JO - Сорбционные и хроматографические процессы

JF - Сорбционные и хроматографические процессы

SN - 1680-0613

IS - 4

M1 - 4

ER -