Определение видового состава антоцианов цветков Сatharanthus

Translated title of the contribution: Determination of catharanthus flower anthocyanins

V.I. Deineka, Yaroslava Kulchenko, A.N. Sidorov, I.P. Blinova, S.M. Varushkina, L.A. Deineka, Thi Ngoc Anh Vu

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Using the reversed phase high performance liquid chromatography with diode-array and massspectrometric (in ESI mode) detection, a composition of Catharanthus roseum petals was established. After the acid hydrolysis, all five anthocyanidins were found to be different comparing to the ordinary anthocyanidins from Vitis vinifera fruits. The anthocyanins were elucidated to be 7-O-methyl derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin by the analysis of retention in RP HPLC, mass- and UV-visible spectra.
The anthocyanins were characterized with UV-visible spectra, having the same fixtures as the set of nonmethylated (in position 7) anthocyanin with hypsochromic (4 nm) of spectral maxima. The absorption bands for 7-methylcyanidin and 7-methylpeonidin aglycons and derivatives were indistinguishable while for the set of 7-methydelphinidin, 7-methypetunidin and 7-methylmalvidine (hirsutidin) a consecutive shift of absorption maxima
by approximately 1 – 1.5 nm was found. The same was true for non-methylates at position 7 derivatives. The analysis of retention of anthocyanins of the flowers including the comparison with the retention of Mangifera indica skin anthocyanins, mass- and UV-visible spectra indicated that a minor set of anthocyanins included two sets of derivatives. The minor compounds were found to be 3-galactosides for samples under investigation, while the set of the major anthocyanins was represented by 3-rhamnosylgalactosides. Indeed, though through mass-spectra it was not possible to differentiate 3-rhamnosylgalatosides and 3-(p-coumaroylgalactosides) because of m/z coincidence, the retention difference between the two found anthocyanins sets as well as UV-visible spectra excluded the latter type of derivatives.
Original languageRussian
Pages (from-to)103-109
JournalАналитика и контроль
Volume23
Issue number1
DOIs
Publication statusPublished - 2019

Fingerprint

Anthocyanins
Derivatives
delphinidin
Galactosides
High performance liquid chromatography
Fruits
Absorption spectra
Hydrolysis
Skin
Diodes
Acids

Keywords

  • mass-spectrometric detection
  • anthocyanins
  • uv-vis spectra
  • 3-phamnosylgalactosides
  • 3-galactosides
  • peonidin
  • malvidin
  • petunidin
  • cyanidin
  • 7-O-methyl derivatives of delphinidin

Cite this

Deineka, V.I. ; Kulchenko, Yaroslava ; Sidorov, A.N. ; Blinova, I.P. ; Varushkina, S.M. ; Deineka, L.A. ; Vu, Thi Ngoc Anh. / Определение видового состава антоцианов цветков Сatharanthus. In: Аналитика и контроль. 2019 ; Vol. 23, No. 1. pp. 103-109.
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author = "V.I. Deineka and Yaroslava Kulchenko and A.N. Sidorov and I.P. Blinova and S.M. Varushkina and L.A. Deineka and Vu, {Thi Ngoc Anh}",
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Определение видового состава антоцианов цветков Сatharanthus. / Deineka, V.I.; Kulchenko, Yaroslava; Sidorov, A.N.; Blinova, I.P.; Varushkina, S.M.; Deineka, L.A.; Vu, Thi Ngoc Anh.

In: Аналитика и контроль, Vol. 23, No. 1, 2019, p. 103-109.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Определение видового состава антоцианов цветков Сatharanthus

AU - Deineka, V.I.

AU - Kulchenko, Yaroslava

AU - Sidorov, A.N.

AU - Blinova, I.P.

AU - Varushkina, S.M.

AU - Deineka, L.A.

AU - Vu, Thi Ngoc Anh

PY - 2019

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N2 - Using the reversed phase high performance liquid chromatography with diode-array and massspectrometric (in ESI mode) detection, a composition of Catharanthus roseum petals was established. After the acid hydrolysis, all five anthocyanidins were found to be different comparing to the ordinary anthocyanidins from Vitis vinifera fruits. The anthocyanins were elucidated to be 7-O-methyl derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin by the analysis of retention in RP HPLC, mass- and UV-visible spectra.The anthocyanins were characterized with UV-visible spectra, having the same fixtures as the set of nonmethylated (in position 7) anthocyanin with hypsochromic (4 nm) of spectral maxima. The absorption bands for 7-methylcyanidin and 7-methylpeonidin aglycons and derivatives were indistinguishable while for the set of 7-methydelphinidin, 7-methypetunidin and 7-methylmalvidine (hirsutidin) a consecutive shift of absorption maximaby approximately 1 – 1.5 nm was found. The same was true for non-methylates at position 7 derivatives. The analysis of retention of anthocyanins of the flowers including the comparison with the retention of Mangifera indica skin anthocyanins, mass- and UV-visible spectra indicated that a minor set of anthocyanins included two sets of derivatives. The minor compounds were found to be 3-galactosides for samples under investigation, while the set of the major anthocyanins was represented by 3-rhamnosylgalactosides. Indeed, though through mass-spectra it was not possible to differentiate 3-rhamnosylgalatosides and 3-(p-coumaroylgalactosides) because of m/z coincidence, the retention difference between the two found anthocyanins sets as well as UV-visible spectra excluded the latter type of derivatives.

AB - Using the reversed phase high performance liquid chromatography with diode-array and massspectrometric (in ESI mode) detection, a composition of Catharanthus roseum petals was established. After the acid hydrolysis, all five anthocyanidins were found to be different comparing to the ordinary anthocyanidins from Vitis vinifera fruits. The anthocyanins were elucidated to be 7-O-methyl derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin by the analysis of retention in RP HPLC, mass- and UV-visible spectra.The anthocyanins were characterized with UV-visible spectra, having the same fixtures as the set of nonmethylated (in position 7) anthocyanin with hypsochromic (4 nm) of spectral maxima. The absorption bands for 7-methylcyanidin and 7-methylpeonidin aglycons and derivatives were indistinguishable while for the set of 7-methydelphinidin, 7-methypetunidin and 7-methylmalvidine (hirsutidin) a consecutive shift of absorption maximaby approximately 1 – 1.5 nm was found. The same was true for non-methylates at position 7 derivatives. The analysis of retention of anthocyanins of the flowers including the comparison with the retention of Mangifera indica skin anthocyanins, mass- and UV-visible spectra indicated that a minor set of anthocyanins included two sets of derivatives. The minor compounds were found to be 3-galactosides for samples under investigation, while the set of the major anthocyanins was represented by 3-rhamnosylgalactosides. Indeed, though through mass-spectra it was not possible to differentiate 3-rhamnosylgalatosides and 3-(p-coumaroylgalactosides) because of m/z coincidence, the retention difference between the two found anthocyanins sets as well as UV-visible spectra excluded the latter type of derivatives.

KW - anthocyanine

KW - mass-spectrometric detection

KW - anthocyanins

KW - uv-vis spectra

KW - 3-phamnosylgalactosides

KW - 3-galactosides

KW - peonidin

KW - malvidin

KW - petunidin

KW - cyanidin

KW - 7-O-methyl derivatives of delphinidin

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DO - 10.15826/analitika.2019.23.1.001

M3 - Article

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EP - 109

JO - Аналитика и контроль

JF - Аналитика и контроль

SN - 2073-1450

IS - 1

ER -