Chromatographic behavior of petanin in reversed-phase HPLC in mobile phases with a wide range of pH

V.I. Deineka, Yaroslava Kulchenko, L.A. Deineka

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The chromatographic behavior of petanin, petunidin-3-O-[6-O-(4-O-(E)-para-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyranoside]-5-O-β-glucopyranoside, is studied for the first time under conditions of reversed-phase high performance liquid chromatography in mobile phases with different pH values. The relationship between chromatographic behavior (retention time and peak efficiency) and transitions between different forms of anthocyanins is discussed. Analysis of the data obtained in the 2 to 6.5 range of mobile pH phases, the absorption spectra of petanin, and the results from studying the effect of adding tetrabutylammonium bromide to the mobile phase shows that increasing the pH results in the formation of uncharged and anionic forms, in addition to the pseudobase form.
Original languageEnglish
Pages (from-to)572-576
Journal Russian journal of physical chemistry A
Volume93
Issue number181
Publication statusPublished - 2019

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Anthocyanins
High performance liquid chromatography
Absorption spectra
petunidin
tetrabutylammonium

Keywords

  • anthocyanins
  • ph
  • hplc
  • quinoid structures
  • flavin form

Cite this

@article{f40f917072254586b16bcc24b8f53a4d,
title = "Chromatographic behavior of petanin in reversed-phase HPLC in mobile phases with a wide range of pH",
abstract = "The chromatographic behavior of petanin, petunidin-3-O-[6-O-(4-O-(E)-para-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyranoside]-5-O-β-glucopyranoside, is studied for the first time under conditions of reversed-phase high performance liquid chromatography in mobile phases with different pH values. The relationship between chromatographic behavior (retention time and peak efficiency) and transitions between different forms of anthocyanins is discussed. Analysis of the data obtained in the 2 to 6.5 range of mobile pH phases, the absorption spectra of petanin, and the results from studying the effect of adding tetrabutylammonium bromide to the mobile phase shows that increasing the pH results in the formation of uncharged and anionic forms, in addition to the pseudobase form.",
keywords = "anthocyanine, anthocyanins, ph, hplc, quinoid structures, flavin form",
author = "V.I. Deineka and Yaroslava Kulchenko and L.A. Deineka",
year = "2019",
language = "English",
volume = "93",
pages = "572--576",
journal = "Russian journal of physical chemistry A",
number = "181",

}

Chromatographic behavior of petanin in reversed-phase HPLC in mobile phases with a wide range of pH. / Deineka, V.I.; Kulchenko, Yaroslava; Deineka, L.A.

In: Russian journal of physical chemistry A, Vol. 93, No. 181, 2019, p. 572-576.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Chromatographic behavior of petanin in reversed-phase HPLC in mobile phases with a wide range of pH

AU - Deineka, V.I.

AU - Kulchenko, Yaroslava

AU - Deineka, L.A.

PY - 2019

Y1 - 2019

N2 - The chromatographic behavior of petanin, petunidin-3-O-[6-O-(4-O-(E)-para-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyranoside]-5-O-β-glucopyranoside, is studied for the first time under conditions of reversed-phase high performance liquid chromatography in mobile phases with different pH values. The relationship between chromatographic behavior (retention time and peak efficiency) and transitions between different forms of anthocyanins is discussed. Analysis of the data obtained in the 2 to 6.5 range of mobile pH phases, the absorption spectra of petanin, and the results from studying the effect of adding tetrabutylammonium bromide to the mobile phase shows that increasing the pH results in the formation of uncharged and anionic forms, in addition to the pseudobase form.

AB - The chromatographic behavior of petanin, petunidin-3-O-[6-O-(4-O-(E)-para-coumaroyl-O-α-rhamnopyranosyl)-β-glucopyranoside]-5-O-β-glucopyranoside, is studied for the first time under conditions of reversed-phase high performance liquid chromatography in mobile phases with different pH values. The relationship between chromatographic behavior (retention time and peak efficiency) and transitions between different forms of anthocyanins is discussed. Analysis of the data obtained in the 2 to 6.5 range of mobile pH phases, the absorption spectra of petanin, and the results from studying the effect of adding tetrabutylammonium bromide to the mobile phase shows that increasing the pH results in the formation of uncharged and anionic forms, in addition to the pseudobase form.

KW - anthocyanine

KW - anthocyanins

KW - ph

KW - hplc

KW - quinoid structures

KW - flavin form

M3 - Article

VL - 93

SP - 572

EP - 576

JO - Russian journal of physical chemistry A

JF - Russian journal of physical chemistry A

IS - 181

ER -