Chromatographic behavior of anthocyanins on a C10CN stationary phase

V.I. Deineka, Yaroslava Kulchenko, Belgorod University

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The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin-
3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retention
of anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography.
Original languageEnglish
Pages (from-to)1233-1238
JournalJournal of Analytical Chemistry
Issue number12
Publication statusPublished - 2017


  • effect of anthocyanin structure
  • selectivity of separation
  • anthocyanins
  • C10CN stationary phase
  • hplc


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