Chromatographic behavior of anthocyanins on a C10CN stationary phase

V.I. Deineka, Yaroslava Kulchenko, L.A. Deineka

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin-
3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retention
of anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography.
Original languageEnglish
Pages (from-to)1233-1238
JournalJournal of analytical chemistry
Volume72
Issue number12
Publication statusPublished - 2017

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Anthocyanins
Chromatography

Keywords

  • effect of anthocyanin structure
  • selectivity of separation
  • anthocyanins
  • C10CN stationary phase
  • hplc

Cite this

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title = "Chromatographic behavior of anthocyanins on a C10CN stationary phase",
abstract = "The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin-3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retentionof anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography.",
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language = "English",
volume = "72",
pages = "1233--1238",
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Chromatographic behavior of anthocyanins on a C10CN stationary phase. / Deineka, V.I.; Kulchenko, Yaroslava; Deineka, L.A.

In: Journal of analytical chemistry, Vol. 72, No. 12, 2017, p. 1233-1238.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Chromatographic behavior of anthocyanins on a C10CN stationary phase

AU - Deineka, V.I.

AU - Kulchenko, Yaroslava

AU - Deineka, L.A.

PY - 2017

Y1 - 2017

N2 - The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin-3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retentionof anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography.

AB - The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin-3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retentionof anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography.

KW - anthocyanine

KW - effect of anthocyanin structure

KW - selectivity of separation

KW - anthocyanins

KW - C10CN stationary phase

KW - hplc

M3 - Article

VL - 72

SP - 1233

EP - 1238

JO - Journal of analytical chemistry

JF - Journal of analytical chemistry

SN - 1608-3199

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ER -