Aerobic exercise increases post-exercise exogenous protein oxidation in healthy young males

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The capacity to utilize ingested protein for optimal support of protein synthesis and lean body mass is described within the paradigm of anabolic competence. Protein synthesis can be stimulated by physical exercise, however, it is not known if physical exercise affects post-exercise protein oxidation. Characterization of the driving forces behind protein oxidation, such as exercise, can contribute to improved understanding of whole body protein metabolism. The purpose of this study is to determine the effect of two levels of aerobic exercise intensity on immediate post-exercise exogenous protein oxidation. Sixteen healthy males with a mean (SD) age of 24 (4) years participated. The subjects' VO2-max was estimated with the Åstrand cycling test. Habitual dietary intake was assessed with a three-day food diary. Exogenous protein oxidation was measured by isotope ratio mass spectrometry. These measurements were initiated after the ingestion of a 30 g 13C-milk protein test drink that was followed by 330 minutes breath sample collection. On three different days with at least one week in between, exogenous protein oxidation was measured: 1) during rest, 2) after 15 minutes of aerobic exercise at 30% of VO2-max (moderate intensity), and 3) after 15 minutes of aerobic exercise at 60% of VO2-max (vigorous intensity). After vigorous intensity aerobic exercise, 31.8%±8.0 of the 30 g 13C-milk protein was oxidized compared to 26.2%±7.1 during resting condition (p = 0.012), and 25.4%±7.6 after moderate intensity aerobic exercise compared to resting (p = 0.711). In conclusion, exogenous protein oxidation is increased after vigorous intensity aerobic exercise which could be the result of an increased protein turnover rate.

Original languageEnglish
JournalPLOS one
Volume14
Issue number11
DOIs
Publication statusPublished - 2019

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exercise
Exercise
oxidation
Oxidation
Proteins
proteins
Milk Proteins
protein metabolism
dairy protein
protein synthesis
Diet Records
lean body mass
food records
body protein
Metabolism
Isotopes
Mental Competency
Mass spectrometry
food intake
isotopes

Keywords

  • protein oxidation

Cite this

@article{0c6e16591e884064baf411bb63551799,
title = "Aerobic exercise increases post-exercise exogenous protein oxidation in healthy young males",
abstract = "The capacity to utilize ingested protein for optimal support of protein synthesis and lean body mass is described within the paradigm of anabolic competence. Protein synthesis can be stimulated by physical exercise, however, it is not known if physical exercise affects post-exercise protein oxidation. Characterization of the driving forces behind protein oxidation, such as exercise, can contribute to improved understanding of whole body protein metabolism. The purpose of this study is to determine the effect of two levels of aerobic exercise intensity on immediate post-exercise exogenous protein oxidation. Sixteen healthy males with a mean (SD) age of 24 (4) years participated. The subjects' VO2-max was estimated with the {\AA}strand cycling test. Habitual dietary intake was assessed with a three-day food diary. Exogenous protein oxidation was measured by isotope ratio mass spectrometry. These measurements were initiated after the ingestion of a 30 g 13C-milk protein test drink that was followed by 330 minutes breath sample collection. On three different days with at least one week in between, exogenous protein oxidation was measured: 1) during rest, 2) after 15 minutes of aerobic exercise at 30{\%} of VO2-max (moderate intensity), and 3) after 15 minutes of aerobic exercise at 60{\%} of VO2-max (vigorous intensity). After vigorous intensity aerobic exercise, 31.8{\%}±8.0 of the 30 g 13C-milk protein was oxidized compared to 26.2{\%}±7.1 during resting condition (p = 0.012), and 25.4{\%}±7.6 after moderate intensity aerobic exercise compared to resting (p = 0.711). In conclusion, exogenous protein oxidation is increased after vigorous intensity aerobic exercise which could be the result of an increased protein turnover rate.",
keywords = "protein oxidation, proteinen",
author = "Reckman, {Gerlof A.R.} and Navis, {Gerjan J.} and Krijnen, {Wim P.} and Vonk, {Roel J.} and Harri{\"e}t Jager-Wittenaar and {van der Schans}, {Cees P}",
year = "2019",
doi = "10.1371/journal.pone.0225803",
language = "English",
volume = "14",
journal = "PLOS one",
issn = "1932-6203",
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Aerobic exercise increases post-exercise exogenous protein oxidation in healthy young males. / Reckman, Gerlof A.R.; Navis, Gerjan J.; Krijnen, Wim P.; Vonk, Roel J.; Jager-Wittenaar, Harriët; van der Schans, Cees P.

In: PLOS one, Vol. 14, No. 11, 2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Aerobic exercise increases post-exercise exogenous protein oxidation in healthy young males

AU - Reckman, Gerlof A.R.

AU - Navis, Gerjan J.

AU - Krijnen, Wim P.

AU - Vonk, Roel J.

AU - Jager-Wittenaar, Harriët

AU - van der Schans, Cees P

PY - 2019

Y1 - 2019

N2 - The capacity to utilize ingested protein for optimal support of protein synthesis and lean body mass is described within the paradigm of anabolic competence. Protein synthesis can be stimulated by physical exercise, however, it is not known if physical exercise affects post-exercise protein oxidation. Characterization of the driving forces behind protein oxidation, such as exercise, can contribute to improved understanding of whole body protein metabolism. The purpose of this study is to determine the effect of two levels of aerobic exercise intensity on immediate post-exercise exogenous protein oxidation. Sixteen healthy males with a mean (SD) age of 24 (4) years participated. The subjects' VO2-max was estimated with the Åstrand cycling test. Habitual dietary intake was assessed with a three-day food diary. Exogenous protein oxidation was measured by isotope ratio mass spectrometry. These measurements were initiated after the ingestion of a 30 g 13C-milk protein test drink that was followed by 330 minutes breath sample collection. On three different days with at least one week in between, exogenous protein oxidation was measured: 1) during rest, 2) after 15 minutes of aerobic exercise at 30% of VO2-max (moderate intensity), and 3) after 15 minutes of aerobic exercise at 60% of VO2-max (vigorous intensity). After vigorous intensity aerobic exercise, 31.8%±8.0 of the 30 g 13C-milk protein was oxidized compared to 26.2%±7.1 during resting condition (p = 0.012), and 25.4%±7.6 after moderate intensity aerobic exercise compared to resting (p = 0.711). In conclusion, exogenous protein oxidation is increased after vigorous intensity aerobic exercise which could be the result of an increased protein turnover rate.

AB - The capacity to utilize ingested protein for optimal support of protein synthesis and lean body mass is described within the paradigm of anabolic competence. Protein synthesis can be stimulated by physical exercise, however, it is not known if physical exercise affects post-exercise protein oxidation. Characterization of the driving forces behind protein oxidation, such as exercise, can contribute to improved understanding of whole body protein metabolism. The purpose of this study is to determine the effect of two levels of aerobic exercise intensity on immediate post-exercise exogenous protein oxidation. Sixteen healthy males with a mean (SD) age of 24 (4) years participated. The subjects' VO2-max was estimated with the Åstrand cycling test. Habitual dietary intake was assessed with a three-day food diary. Exogenous protein oxidation was measured by isotope ratio mass spectrometry. These measurements were initiated after the ingestion of a 30 g 13C-milk protein test drink that was followed by 330 minutes breath sample collection. On three different days with at least one week in between, exogenous protein oxidation was measured: 1) during rest, 2) after 15 minutes of aerobic exercise at 30% of VO2-max (moderate intensity), and 3) after 15 minutes of aerobic exercise at 60% of VO2-max (vigorous intensity). After vigorous intensity aerobic exercise, 31.8%±8.0 of the 30 g 13C-milk protein was oxidized compared to 26.2%±7.1 during resting condition (p = 0.012), and 25.4%±7.6 after moderate intensity aerobic exercise compared to resting (p = 0.711). In conclusion, exogenous protein oxidation is increased after vigorous intensity aerobic exercise which could be the result of an increased protein turnover rate.

KW - protein oxidation

KW - proteinen

U2 - 10.1371/journal.pone.0225803

DO - 10.1371/journal.pone.0225803

M3 - Article

C2 - 31765432

VL - 14

JO - PLOS one

JF - PLOS one

SN - 1932-6203

IS - 11

ER -